Detection of Virulence Factors hla and hlb of Staphylococcus aureus using PCR Technique


Thirty isolates were collected from patients sputum and throat swabs who admitted at Teaching General Hospital in Hilla city during a period of two months lasting from (Febraury to March 2014). The morphological characterization and biochemical reactions showed 18 isolates diagnosed as Staphylococcus aureus, of which obtain only six isolates, two from sputum samples (25%) and four from throat swabs (0.4%) have hlb gene and only four isolates, two from sputum samples(25%) and two from throat swabs (0.2%) have hla gene using PCR techniques.