Effect salicylic acid on producing volatile oils from Garlic Allium sativum L. in vitro

Abstract

The research was conducted to study the effect of adding different concentrations of Salicylic acid (0,50,100,150,200) mg/ l in the production of some secondary metabolic compounds of plant(garlic) Allium sativum L. Quantitative and qualitative analysis of secondary metabolites were estimated by using( HPLC ). Garlic cloves were culture on MS media supplemented with 2mg/l of 2,4-D, 0.5mg/l of NAA and 1mg/l of BA for callus induction. Adding salicylic acid (100 mg/l) cause in significant increase of fresh weight 734mg and volatile oil in callus extract compared.