EFFECT OF OOCYTE HARVESTING METHOD ON THE RECOVERY AND IN VITRO MATURATION IN SHEEP

Abstract

Two different methods were used for harvesting the oocytes of ovine ovaries. The aim was to determined an efficient method for oocyte harvesting and its effect on subsequent in vitro maturation. The average percentage of oocytes recovered was significantly (p<0.05) higher by slicing (84.60 ± 1.01) than by aspiration method (67.60 ± 1.37). While the percentage of good quality mature oocytes was higher in the aspiration method (40.57 ± 0.90) as compared with slicing methods (37.40 ± 0.27). There was a significant difference (p<0.05) between two methods. It was concluded from this study that the method of harvesting oocytes from ovaries in slaughter house affect the recovery and the maturation of the oocyte in sheep.