Evaluation of Modified Oxidase, Cover-Glass Test, Antibiotic Sensitivity for Differentiation of Staphylococcus spp. from Other Related Genera

Abstract

To comparison between modified oxidase, cover-glass, antibiotic sensitivity (Nitrofurantoin, Furazolidone) to evaluate which of them are most useful for differentiation Staphylococcus spp. from other related genera. A total of 115 clinical isolates out of them 38 isolates belongs to Staphylococcus spp. were collected from pus, urine and blood cultures of patients for both gender from period March to July 2012 at Al-Salam General Teaching Hospital in Mosul city. All isolates were diagnosed and identified classically and using API test. The result indicated that Imipenem is highly effective against Staphylococcus spp. and all isolates were negative to modified oxidase test except one isolate. All other isolates were given good growth under cover-glass test except one. 67.5% of isolates were resistant to Nitrofurantoin and 32.5% were sensitive but 21.6% of isolates were resistant to Furazolidone and 78.4% were sensitive. The above tests are the most tests in differentiation between Staphylococcus spp. and other related Genera.