TY - JOUR ID - TI - Effect of Hygromycin B Antibiotic Produced by Streptomyces Isolates on some Pathogenic Bacteria AU - Abdul Wahid B. Al-Shaibani AU - Rabah N. Jabbar AU - Zahraa Abdulmunim Sharba PY - 2016 VL - 19 IS - 2 SP - 105 EP - 110 JO - Al-Nahrain Journal of Science مجلة النهرين للعلوم SN - 26635453 26635461 AB - To select the most efficient isolate in production of the antimicrobial agent Hygomycin B, 53 Streptomyces soil isolates were examined. DNA of these isolates was extracted and screened by Polymerase Chain Reaction (PCR) technique for the presence of Hygomycin B gene which is responsible for production of such agent. Among the 53 isolates, only three gave positive results for the existence of this gene when the three isolates were evaluated for their antimicrobial activity, an isolate symbolled Streptomyces “10cm/35pigment” was the superior by giving the highest activity against four of the seven test microorganisms (Proteus mirabilis, Staph. aureus, Sal. typhi and Sacch. cerevisiae) used in the study. Their recorded inhibition zone diameters were 70, 55, 33 and 38 mm, respectively. After subjection of the superior isolate to the cultural, microscopic and biochemical characterization, it was identified as Streptomyces hygroscopicus.

لأختيار اكثر عزله منتجه لمضاد الـHygomycin B ، ـتم اختبار 21 عزله وذلك بعزل الحامض النووي الـ DNA والبحث عن المورث المسؤل عن انتاج المضاد بأستخدام بادءات متخصصه في تقنية الــــ PCR .من هذه الــــ21 عزله، فقط ثلاث عزلات اثبتت وجود الجين وتم قياس الفعاليه الحيويه لهذه العزلات، العزله بالرمز“10cm/ 35pigment” تفوقت على باقي العزلات باعطاء اعلة فعاليه حيويه ضد اربعه من سبع احياء مجهريه تحت الاختبار(Proteus mirabilis, Staph. aureus, Sal. typhi and Sacch. Cerevisiae) استخدمت في هذه الدراسه، حيث اعطت منطقة تثبيط 70, 55, 33 and 38 mm, بالتعاقـــب بعـــــد اخضــــاع العزلــــه المتفوقــــه لفحوصـــــاتمظهريه ومجهريـــه وكيمــــوحيويـــــه شخصت علــــى انهـــــــا Streptomyces hygroscopicus. ER -