@Article{, title={An Evaluation of Antimicrobial Efficacy of Steralium, co+steralium, and 5% Sodium Hypochlorite against Enterococcus Faecalis Biofilm Formed on Tooth Substrate: (An in Vitro Study)}, author={Ayad M. Al-Kadhi}, journal={Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد}, volume={28}, number={1}, pages={11-16}, year={2016}, abstract={Background: Enterococcus faecalis is emerging as an important endodontic pathogen, which can persist in theenvironment for extended periods after treatment and may cause endodontic failure. It is known to producebiofilms, a community of bacteria enclosed within a protective polymeric matrix. This study aimed to establishwhether the biofilm formation by Enterococcus faecalis can be inhibited with steralium, co+steralium, and 5%sodium hypochlorite in the root surface environment.Materials and Methods: Extracted human teeth were biomechanically prepared, vertically sectioned, placed in thetissue culture wells exposing the root canal surface to E. faecalis to form a biofilm. At the end of the 3rd and 6thweeks, all groups were treated for 10 minutes with the previously mentioned tested solutions and control and wereanalyzed qualitatively and quantitatively.Results: Qualitative assay with 3-weeks biofilm showed a complete inhibition of bacterial growth with co+steraliumand NaOCl, except steralium and saline, which showed presence of bacterial growth. In quantitative analysis,steralium and saline- treated tooth samples have 57.74 ± 5.39* CFU/ml., 140.71 ± 8.49 CFU/ml. respectively.Qualitative assay with 6-week biofilm showed growth when treated with steralium and saline treated tooth sampleshave 346.0 ± 78.88* CFU/ml., 127.18 ± 17.84 CFU/ml. respectively whereas co+steralium and NaOCl has showncomplete inhibition.Conclusions: co+steralium and 5% sodium hypochlorite showed maximum antibacterial activity against E. Faecalisbiofilm formed on tooth substrate. co+steralium showed statistically significant antibacterial activity. The use ofco+steralium as a root canal irrigant might prove to be advantageous considering the several undesirablecharacteristics of NaOCl

} }