TY - JOUR ID - TI - Histological evaluation of local application of collagen I and /or vascular endothelial growth factor (An experimental study in rats) AU - Rafal Rashid رفل رشيد AU - Athraa Y. Al-Hijazi عذراء يحيى الحجازي PY - 2015 VL - 27 IS - 3 SP - 79 EP - 84 JO - Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد SN - 18171869 23115270 AB - Background: The study was designed to evaluate the effect of local application of exogenous VEGF/collagen Iseparately and as a combination in socket healing. Sixty male Albino Wistar rats were subjected for a surgical toothextraction of upper 1st molar of both sides (right side was considered as experimental site, while left be the controlone, treated with 1μL of normal saline). The rats were scarified at 3, 7, 14, 28 days post extraction. Socket healing washistologically examined with immunohistochemistochemical localization of ALP&FGF2.Materials and Method: Sixty male Albino Wistar rats were subjected for a surgical tooth extraction of upper 1stmolarof both sides (right side was considered as experimental site, while left be the control one). The animals were dividedinto following groups according to the applicable of biomaterials.A. Control group the tooth socket treated with 1μL of normal salineB. Experimental group includes• Group I contains (20) rats, the tooth socket treated with 1μL of VEGF• Group II contains (20) rats the tooth socket treated with 1μL of collagen type I• Group III contains (20) rats, the tooth socket treated with 1μL of a combination of VEGF and collagen I.Results: At 28 days all groups show re-epithelization but in different thickness, and with newly bone apposition andwith different maturity. For positive cells expressed ALP, VEGF group records a high mean values at 3, 14, 28 daysperiods and with high differences in comparison to other groups while control group reports a high mean value at 7days. For positive cells expressed FGF2. Control group illustrates a high record for the mean of positive cellsexpressed FGF2 at 3,7days periods and with high differences in comparison to other groups, while combinationgroup reports a high mean value at 14 days .Conclusion: Results, high lighted on the effect of local application of VEGF in extracted tooth socket that facilitateepithelization, while combination of (collagen and VEGF) shows a high mineralization zone

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