TY - JOUR ID - TI - Assessment and correlation of Anti-sperm antibodies to sperm parameters in normozoospermic men AU - Muhammad-Baqir M-R Fakhirldin AU - Zaidoon H. Jabbar PY - 2013 VL - 3 IS - 6 SP - 21 EP - 25 JO - Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم SN - 26166984 22180265 AB - Background:The immune infertility caused by anti-sperm antibodies (ASAs) represented about 1020%- of infertility among the couples, the ASAs interfere with sperm parameters such as the sperm motility and sperm ability to penetrate cervical mucus, sperm-oocyte binding, and fertilization and embryo developments.Objectives:The present study designed to assess semen analysis, presence of ASAs and DNA fragmentation index as well as correlation within these parameters in normzoospermic Iraqi subjectsPatients, Materials and Methods:A total number of Iraqi subjects (116) with range of age (2051-) years and their mean duration of infertility (4.70 ± 2.77). Seminal fluidforMacroscopicandMicroscopicassessmentsdoneaccording to WHO 2010 criteria. The mixed agglutination reaction (MAR) test used to assess their ASAs in semen (direct method), seminal plasma and serum (indirect method); for the both IgG and IgA classes of antibodies and their distribution among different parts of spermatozoa as percentage.Results:The mean of IgG using direct method was (23.881.75±) and for indirect detection method the mean was (27.412.41±) with no significantdifference(P<0.05)betweenthetwomethods.Whilethe direct method for IgA detection mean (14.46 ±1.76) and the mean for indirect method of detection (6.860.39±) and there was significantdifference(P>0.05)betweenthetwomethods.In addition to that, the distribution of IgG and IgA detected by direct method on sperm parts showed no significancedifferenceexceptforthespermtail;whiletheindirectmethodforIgAand IgG showed significantdifferenceindistributiononspermmid-piece.Conclusion:The correlation among sperm parameters and Immunoglobulin using direct and indirect methods showing variations in significancealsointhecorrelationbetweenthedirectandindirect methods according to distributions on sperm surface

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