@Article{, title={Inhibition of Binding of Helicobacter pylori to Tissue Culture Cells by Anti-Lipopolysaccharides Monoclonal Antibodies}, author={MaanAbul-Milh*}, journal={Muthanna Journal of Pure Science (MJPS) مجلة المثنى للعلوم الصرفة}, volume={2}, number={1}, pages={1-23}, year={2014}, abstract={Binding of 35[S]- methionine-labeled Helicobacter pylori to tissue culturewas carried out using human colon carcinoma epithelial cells (Caco-2),and human adenocarcinoma tissue culture cells (AGS). In this research,clinical isolates (smooth) as well as type culture collection (rough) strainswere used in binding and inhibition of the binding study of H. pylori totissue cultures using monoclonal antibodies (MAbs) raised againstbacterial lipopolysaccharides (LPS). The task of the work was todiscriminate and evaluate the role of LPS as bacterial adhesin in thebinding as well as the specificity of the binding of various H. pylori strainsto cell lines. Binding of H. pylori, clinical isolate Hel 305(smoothstrain),to Caco-2 cells was strongly inhibitedby species-specific MAbsraised against Hel 305-LPS antigen. It was also recognized that inhibitionof binding of Hel 305 strain to Caco-2 cells was high effective when usingspecies-specific MAbs in comparison with MAbs raised against LPS fromtype culture collection CCUG 17874 (rough strain). In the same manner,binding of rough CCUG 17874 strain to Caco-2 cell line was inhibited byspecies-specific LPS MAbs stronger than with MAbs raised against Hel305 LPS. In both overlay cases, no significant inhibition with other LPSMAbs of different origins was detected, after bacterial incubation withMAbs raised against LPSs from other tested H. pylori strains.

} }