TY - JOUR ID - TI - DETECTION OF BACILLUS THURINGIENSIS CRYSTAL PROTEIN CRY51AA1 INTOXICATION (IN VIVO) USING ZEBRAFISH MODEL AU - Ali B.T. Aldeewan PY - 2017 VL - 16 IS - 2 SP - 26 EP - 35 JO - Basrah Journal of Veterinary Research مجلة البصرة للابحاث البيطرية SN - 18138497 24108456 AB - Bacillus thuringiensis is a ubiquitous a gram-positive, anaerobic, spore-formingbacterium, produces various proteins (toxins) during the stationary and vegetative phaseof its growth cycle. Some of these proteins belonging to this group showed weaksimilarities to ß-barrel pore-forming toxic proteins, such as the cytotoxins ofPseudomonas aeruginosa, the epsilon toxin of Clostridium perfringens, alpha-toxin ofClostridium septicum. In the present study, the intoxication effects of Cry51Aa1 proteinproduced by B.thuringiensis F14-1 strain was evaluated in vivo, in order to investigate theability of activated Cry51Aa1 to make a change in size or functions of zebrafishcardiovascular system and induces toxicity in adult zebrafish. Using in vivo imaging weobserved that Cry51Aa1 has no scientific effect on cardiac function and development ofzebra fish embryo or have toxic effect on adult in spite of use high concentration, so it issafe to use and a potentially effective agent in breast cancer therapy.

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