TY - JOUR ID - TI - 5-EVALUATION OF INTRAYOLK SAC INOCULATION OF INFECTIOUS BURSAL DISEASE VACCINE ON IMMUNE RESPONSES IN NEWLY HATCHED BROILER CHICKS AU - Harith A. Najem PY - 2018 VL - 17 IS - 1 SP - 52 EP - 62 JO - Basrah Journal of Veterinary Research مجلة البصرة للابحاث البيطرية SN - 18138497 24108456 AB - A total of 60 one-day old Ross broiler chicks were used in this study. The birdswere divided into four groups. The first group A was vaccinated against IBD with a CevacIBD L vaccine at one-day old by intra-yolk sac method (lYS) with a dose of 0.5ml using a(1ml) syringe, whereas the second group B was vaccinated against IBD with the samevaccine at 14 days of age by drinking water method (DW). The third group C and fourthgroup D were considered as a positive and negative control respectively . On day 35, thebirds were weight and killed to collect blood samples and lymphoid organs wereremoved and weight to study the effects of (lYS) rout on body weight , lymphoid organweights and the humeral immune response to IBD vaccine which measured by ELISAtest. The results showed significant differences at (P≤0.05) in body weight of group A, inaddition significant (p<0.05) increase in the all lymphoid organs weight , Bursa offabricius (BF) was 1.643 a ±0.066 in group comparison with other groups which had beenrecorded 1.102 b ±0.022, 0.850 c ±0.067 in group B and C respectively. Group B indicatedthat was a significant decrease at (p<0.05) in the weight of of spleen which was 1.102 b± 0.022 in comparison to control groups. With regard to serological test, bird from groupA recorded higher Ab titer 24038 a± 685 compared to those of other groups , and thisincrement was statically significant (p<0.05), however both group A and B revealed a53significant differences( p <0.05) from the control groups. Generally the results indicatedthat intra-yolk sac method was highly effective rout of IBD vaccine administration

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