TY - JOUR ID - TI - Immunohistochemical expression of VEGF in relation to VEGFR and CD34 in NHL using digital image analysis system AU - Bassam Mohammad Hameed AU - Hind Shaker Aboob AU - Raad Jaber Musa PY - 2016 VL - 5 IS - 1 SP - 19 EP - 31 JO - Iraq Joural of Hematology المجلة العراقية لامراض الدم SN - 20728069 25432702 AB - Background: Lymphoma growth and progression appear to be promoted by at least two distinctangiogenic mechanisms: autocrine stimulation of tumor cells via expression of Vascular EndothelialGrowth Factor(VEGF) and Vascular Endothelial Growth Factor Receptor(VEGFR) by lymphomacells, and paracrine influences of the proangiogenic tumor microenvironment on local tumorvascularity.Objectives: To assess autocrine effect of VEGF, by studying the correlation of VEGF expressionwith its receptor VEGFR expression in NHL. And to assess paracrine effect of VEGF, by studying thecorrelation of VEGF expression with CD34 expressed on endothelial cells in Non-HodgkinLymphoma (NHL).Materials and Methods: A cross sectional study was designed. A total of 66 bone marrow tissuesamples were included in the study, all diagnosed as having NHL according to working formulation.From each block, 3 sections were taken, and were immunohistochemically stained for CD-34, VEGFand VEGFR. Scoring of Immunohistochemical staining was performed using specialized automatedcellular image analysis system, Digimizer software, version 3.7.0.Results: VEGF Immunohistochemical digital parameters named digital labeling index (DLI) wassignificantly correlated with the followings; VEGFR (DLI) [P =0.042, r =0.324], CD34 stained area(A) [P=0.037, r =0.556]. Also VEGFR (DLI) was significantly correlated with CD34 (A).Conclusion: Autocrine and paracrine effect of VEGF is evident in NHL, as there is positivecorrelation between VEGF expression and VEGFR expression, and as tumor vascularity increase withthe increase in VEGF expression.

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