TY - JOUR ID - TI - Effect of Fusarium graminarum Silver-Nanoparticles on IL-10 and INF-γ Cytokines Levels in the Mice by Leishmania donovani in vivo AU - Sabaa Taher Mohamed , Haydar Hassan Ghadi , Nidaa Mohammed Sulaiman , Sura Basil Kamal , Hassan Ali Aja PY - 2019 VL - 18 IS - 2 SP - 106 EP - 115 JO - Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية SN - 18154794 25207245 AB - Leishmaniasis remains one of the fatal diseases worldwide, and the conventional antileishmanial therapies are toxic and most are expensive. Biological silver nanoparticles possess broad-spectrum antimicrobial activities and could be a future alternative to current antimicrobial agents. In the present study an approach was made to synthesize silver nanoparticles (AgNps) using a Fusarium graminarum fungus. The present study it investigates the efficiency of silver nanoparticles against Leishmania donovani compared with pentostam drug in vivo by measuring the levels of immune cytokines (IL-10 and IFN-γ) in serum infected mice and treatment with AgNPs (0.1 ml / day) and comparisons with pentostam drug (0.01ml / day) after 21 days of treatment. The results showed that the level of IFN-γ in treating with AgNPs increased significantly in third weeks, compared to the pentostam group. When treated with pentostam/AgNPs together, there is a gradual decrease in the level of IFN-γ, compared with negative control. Also a significant increase occurs in the IL-10 level within 21 days when mice were treated with AgNPs compared with pentostam. It could be conclude that silver nanoparticles induce pro and anti-inflammatory cytokines also it safety, nontoxic and has a good anti-parasitic activity, it can be used as antileishmanial drug or can be used as supportive treatment of visceral leishmaniasis.

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