@Article{, title={Molecular Detection of Carbapenemase‑Producing Pseudomonas aeruginosa Isolated from Intensive Care Units of Surgical Specialty Hospital in Erbil City}, author={Soza Tharwat Baban}, journal={Medical Journal of Babylon مجلة بابل الطبية}, volume={17}, number={2}, pages={185-193}, year={2020}, abstract={Background: The emergence and spread of carbapenem‑resistant Pseudomonas aeruginosa (CRPA) is a serious cause of nosocomial infectionsin critically ill patients. Objectives: The aim of this study was to determine the prevalence of CRPA and carriage of class B Metallo‑β‑lactamaseresistant genes in intensive care units (ICUs) from patients with surgical‑site infection or ventilator‑associated pneumonia at a Surgical SpecialtyHospital in Erbil city. Materials and Methods: During 6 months’ study, a total of 80 clinical samples were collected from ICUs. The identificationof P. aeruginosa in clinical specimens was confirmed by polymerase chain reaction (PCR) amplification of a molecular marker–oprL gene.Antimicrobial susceptibility was performed using Vitek‑identification system. Screening of carbapenemase‑producer isolates was confirmedusing Rapidec Carba NP test. CRPA isolates were tested for the presence of metallo‑β‑lactamase (MBL)‑encoding genes, including: blaVIM,blaIMP, and blaNDM by using PCR. Results: A total of 50 (62.5%) P. aeruginosa isolates were identified with antibiotic resistance profile (4% pandrug resistant [PDR], 20% extensively drug resistant [XDR] and 76% multidrug resistant). Twelve (24%) isolates were CRPA positive, in whichthe most prevalent MBL‑encoding gene was blaVIM (58.3%), blaNDM (41.7%), and blaIMP (33.3%). Conclusions: Alarmingly, high prevalence ofCRPA with predominance of MBL‑encoding genes was detected. The XDR and PDR resistance phenotypes have become highly prevalent forthis nosocomial pathogen in ICU patients that may cause a therapeutic impasse. The MBL‑encoding genes were predominant among clinicalisolates of P. aeruginosa. These findings emphasize on adherence to infection prevention and control standard precautions, early detection ofCRPA isolates and development of to effectively reduce the burden of carbapenem resistance.

} }