@Article{, title={Ionizing Radiation Effect and DNA damage in the workers of Al-Tuwaitha Nuclear Site}, author={LinaS.Al-Jameel1* and Rasha S.Ahmed1 and Haidar A.Shamran2}, journal={Iraqi Journal of Science المجلة العراقية للعلوم}, volume={60}, number={12}, pages={2636-2641}, year={2019}, abstract={Single cell gel electrophoresis (SCGE), also called comet assay, is a rapid andsensitive technique used to analyse DNA Fragmentation Index(DFI). This studyaimed to evaluate DNA damage in lymphocytes due to ionizing radiation in workersof Al-Tuwaitha nuclear site, which has been used for nuclear activities and containsa potentially significant amount of radioactive waste.The workers in this site arevulnerable to pollution due to a highly polluted environment of ionizing radiation.Blood samples were collected from 36 workerswho were divided into two groups;8workers without protection and 28workers with protection, in addition to 30 controlsubjects.Alkaline comet assay was applied for analysis and the results indicatedsignificantly higher DNA damage in the workers without protection as comparedwith the workers with protection and control groups.The result also showedsignificant differences between the workers with protection and control groups.Keywords: DNA Fragmentation Index, Comet assay, DNA damage, Ionizingradiation , Al-Tuwaitha nuclear site.

} }