TY - JOUR ID - TI - Treatment isolated fungi from laboratory tools in some Baghdad hospitals by using biosynthesized nanoparticles AU - Rayaheen Mohammed Tamkeen* AU - Rusol M .Al-Bahrani PY - 2019 VL - 60 IS - 8 SP - 1673 EP - 1681 JO - Iraqi Journal of Science المجلة العراقية للعلوم SN - 00672904 23121637 AB - The study aims to biosynthesized of sliver nanoparticle from aqueous extract ofolive leave and evaluate the effectiveness of the synthesis AgNPs against isolatedfungi. The study mediating fifty samples were taken from various tools inlaboratory from five hospitals in Baghdad. Four species of fungi were identifieddepending on the morphological and microscopic characteristics. The most commonisolated fungi based on their frequency ratio were as follows Aspergillus niger87.5%, Aspergillus flavus 62.5%, Aspergillus fumigatus 53.5% and Aspergillusnidulans 37.7%.The Biosynthesis of silver nanoparticle developed a rapid, ecofriendlyand convenient green method for the stable silver nanoparticles (AgNPs)were synthesised with an average diameter of 30 ± 60 nm and like spherical inshape, using the aqueous solution of the Olive tree (Olea europaea) leavesextract.The reaction is carried out at 10-3M of silver nitrate. The AgNPs synthesizedwere confirmed by their change of color to (dark brown-grey). The characterizationwas studied using UV-Visible spectroscopy, Scanning Electron Microscopy (SEM)and Atomic Force Microscopy (AFM). Inhibition effect of AgNPs against fungi hasbeen studied using well diffusion method by studying the effect of differentconcentration (100, 75, 50 and 25). The results revealed that the AgNPs haveconsiderable antifungal activity comparison with alcohol. The obtained resultsindicate that the highest level of inhibition zone was detected at the concentration of100 μg/ml of AgNPs, where the inhibition zones are (23.33 ± 4.41) for A. flavus andthe lowest level of inhibition zone was detected at the concentration 25 μg/ml ofAgNPs ,where the inhibition zones are (6.00 ± 1.15) for A.nidalus. While usingalcohol the highest level of inhibition zone was detected at the concentration of 100μg/ml of Alcohol, where the inhibition zones are (12.33 ± 1.45) for A.nidalus, andthe lowest level of inhibition zone was detected at the concentration 25 μg/ml ofAlcohol ,where the inhibition zones are (4.67± 0.33) for A.flavus.

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