@Article{, title={Effect of L.Carnitine supplementation to in-vitro culture media on maturation of bovine oocytes and embryos development}, author={Ahmed S. Al-Ebady}, journal={Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري}, volume={18}, number={2}, pages={11-19}, year={2019}, abstract={This study was conducted to investigate the role of L.Carnitine in enhancing the developmental progress of in-vitro produced bovine embryos. A total of 794 cumulus-oocyte complexes (COCs) were collected by the slicing method from the genitalia of unknown age and breed slaughtered cows. The COCs were divided into four groups and matured in TCM-199 supplemented with four concentrations of L.Carnitine (0.0mg/ml, 0.3mg/ml, 0.6mg/ml and 1.2mg/ml), a concentration for each group. The results showed that the groups treated with 0.3 and 0.6 mg/ml recorded higher percentages of oocytes maturation (48.98% and 49.20%) respectively with significant (P<0.01) differences compared with control and 1.2mg/ml. Matured oocytes were fertilized by straws of frozen-thawed semen, and the resulted embryos were divided into four groups and cultured in media supplemented with the four concentrations of L.Carnitine (0.0mg/ml, 0.3mg/ml, 0.6mg/ml, and 1.2mg/ml) in order to investigate its effect on embryos developmental progress. Results showed that the concentrations (0.3 and 0.6 mg/ml) gave better percentages of embryo development (18.55% and 17.20%) respectively with significant (P<0.05) differences when compared with control and 1.2mg/ml. In conclusion, supplementation of in-vitro bovine oocytes maturation media and in-vitro embryo development media with 0.3 and 0.6 mg/ml of L.Carnitine improved significantly oocyte maturation and embryo developmental progress.

} }