@Article{, title={Inhibition the Growth of Some Malignant Cells Lines by Alfalfa Aqueous Extract دراسة تأثير المستخلص المائي لنبات الجت في تثبيط نمو بعض الخطوط الخلوية السرطانية}, author={Rafid abdul.wahid.A رافد عبد الواحد عبد الكريم}, journal={Iraqi Journal of Cancer and Medical Genetics المجلة العراقية للسرطان والوراثة الطبية}, volume={3}, number={2}, pages={46-50}, year={2010}, abstract={The aim of the present study was to assess the cytotoxic activity of aqueous extracts of Alfalfa (Medicago sativa) by using (MTT) (colorimetric assay) to measure the cytotoxic activity on two malignant tumor cell lines (AMN3) Ahmed Mohamed Nahi 2003 and (GB) Glioblastoma cell line ,and one type of normal cell line (MEF) MouseEmbryo Fibroblast cell line, through different exposure time (24, 48 ,and 72hrs ) and different concentration ofaqueous extract ; (1000,500,250,125 ,and 62.5, mg/ml), the study was carried out in the Iraqi Center for Cancer andMedical Genetics Research ,The result showed that the aqueous extracts from alfalfa have significant effects P value≤ 0.05 on the growth of AMN3 cell lines and GB malignant cell line in culture in dose and time- dependant manner,Viability of cell lines decreased with time and concentration reaching its lowest value after 72 hrs of treatment withthe highest concentration used (1000 mg/ml ). The results also indicated that GB cell lines were more sensitive to theAlfalfa aqueous extracts as compared with the growth of AMN3 cell lines .From the other side the result showed that there is no significant effect P value ≥ 0.05 of these aqueous extractson the viability of normal cell line (MEF3 cell line). Only the treatment with higher concentration (1000μg/ml) after72 hrs caused significant reduction P value ≤ 0.05 in the (MEF) cell line growth.

} }