@Article{, title={Evaluation of cryoprecipitate as part of The qualityassurance in the Iraqi National Blood Transfusion Centre}, author={Saad Shawqi Mansoor}, journal={Journal of the Faculty of Medicine Baghdad مجلة كلية الطب}, volume={50}, number={2}, pages={211-218}, year={2008}, abstract={Background:- Cryoprecipitate (CRYO) is the cold- precipitated concentration offactor VIII , it is prepared from fresh frozen plasma (FFP) by rapid freezing withinsix hours of collection and thawed slowly between 1 - 6 Cº and removed from thesupernatant . The product contain most of F VIII and part of fibrinogen from theoriginal plasma as well as F XIII Von Willebrand (vWF) and fibronectinAim of the study: -This study is conducted to provide more information aboutsignificant contents of cryoprecipitates in regard to factor VIII, fibrinogen, and vonWillebrand factor as part of the quality assurance in blood transfusion centers and toprovide competent and efficient therapeutic materials to patients with bleedingdisorders.Materials and Methods: -In this study 98 samples were taken from the Iraqi blooddonors at National Blood Transfusion Centre ( NBTC ) within 9 months fromOctober 2005 to the end of June 2006. The samples were arranged in two groups.Group I; 56 random samples of CRYO of different blood groups (beforemodification) were used , of them 28 bags were used for FVIII and 22 bags forfibrinogen measurement . Another 25 bags were used for to measure vWF before andafter processing .Group II-A :- 16 random plasma bags were pooled together and divided into 15bags each containing 200 ml of plasma which were frozen and thawn at ( 1-6 Cº )after 21, 22, and 23 hours.Group II-B :- Another 25 random samples of cryoprecipitates were taken fromNBTC after thawing the plasma for 22 hours and using plasma volume above 150 ml(after modification).The statistical methods used were independent sample T – test and analysis ofvariance (ANOVA).Results: - In group I (n=28 ) only 6 bags (21.4 % ) contain more than 70 units ofFVIII which did not meet the COE criteria , while 18 bags (81.8 %) contain morethan 140 mg of fibrinogen per bag which met the COE criteria.In group II A (n=16) thawing after 22 hours was the optimal time for separation ofFVIII (P=0.027).In group II B (n=25), FVIII and fibrinogen level separated from 200ml and 170 mlof plasma respectively were significantly more than those separated from less than200 and 170 ml respectively (P=0.015).Moreover FVIII and fibrinogen separated from group II Bsamples weresignificantly more than those separated from group I. (P=0.001, P=0.027respectively).

} }