TY - JOUR ID - TI - Medico-Legal Applications of Multiplex STR System to Show Allel Frequencies of D16S 539, D13S317, and D7S820 in Iraqis AU - Hussein Mahmood Gazi AU - Mohammed Shafik Tawfeek AU - Nabeel Ghazi Hashim AU - Hayder Hadi Lazim PY - 2010 VL - 9 IS - 4 SP - 542 EP - 548 JO - Iraqi Postgraduate Medical Journal المجلة العراقية للاختصاصات الطبية SN - 83601608 98932708 AB - ABSTRACT:BACKGROUND:Disputed paternity is one of the most important medico-legal problems in Iraq. In addition toidentification of unknown corpses. Paternity cases are resolved by doing ABO typing and HLAserotyping and both are less accurate than DNA typing. To our knowledge this is the first study of suchtype in Iraq.OBJCTIVE:To show the medico-legal importance of determination of allel frequencies in Iraqi population.SUBJECTS AND METHODS:Whole blood was obtained in EDTA tubes by venepuncture from 38 individuals. The DNA was extractedusing the Wizard Genomic DNA Purification Kit and the quantity was estimated by UV-absorbance. Themultiplex analysis of D16S539, D7S720, and D13S317 was performed using the Gene Print STRMultiplex system. Amplification was performed in eppendorf thermal cycler. The PCR products weretyped by vertical electrophoresis on 0.4 mm thick 7% denaturing polyacrylamide gel and silver staining.RESULTS:In our study the DNA fingerprinting test has high accuracy rate.CONCLUSION:Since the DNA typing is the most accurate method so it can be routinely used as a paternity test, it is theonly test that can meet the increasingly imperative demand to resolve the social and judicial problemsinvolved in paternity suits and other medico-legal problems.

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