@Article{, title={Identification of Enterococcal Species by Polymerase Chain Reaction Technique, with Study of Some Immunological Features}, author={Zainab A. Chabuck Alaa H. Al-Charrakh}, journal={Medical Journal of Babylon مجلة بابل الطبية}, volume={9}, number={4}, pages={824-832}, year={2012}, abstract={Objectives: Detection of genes responsible for identification of enterococci to the species level using PCR, determination of enterococcal serum resistance and phagocytic index.Materials and methods: seven enterococcal isolates were obtained from two hundred urine samples. After identification of enterococcal isolates by biochemical tests, all of them were subjected to PCR technique using species-specific PCR primers for E. faecalis and E. faecium, enterococcal serum resistance and phagocytic index were detected.Result: This study revealed that five isolates (71.43%) reacted with ddlE. faecalis; whereas the remaining (28.57%) isolates reacted with ddlE. faecium. Also, revealed that phagocytic index of two E. faecalis and one E. faecium isolates were 27%, 25% and 19%, respectively, while one E. faecium isolate showed phagocytic index as 8%. Moreover, enterococcal serum resistance showed that E. faecalis was more sensitive to the serum as compared to E. faecium.Conclusion: Enterococcal isolates can't be easily identified to the species level by conventional or traditional methods but it can be easily identified by species-specific PCR primers ddlE. faecalis and ddlE. faecium. Enterococcous faecalis tends to be more sensitive to innate immune parameters as compared to E. faecium.

تضمن البحث دراسة بكتريولوجية وجينية وبعض الصفات المناعية لجنس Enterococcous إذ تم الحصول على 7 عزلات من ضمن 200 عينة ادرار من المرضى.لم يتم تشخيص العزلات إلى مستوى النوع بإستخدام الفحوصات الكيموحيوية, على خلاف الفحص الدقيق والسريع لعزلات Enterococcous spp باستخدام Polymerase chain reaction (PCR) مع البوادئ المتخصصة لتحديد أنواع E. faecalis, E. faccium, وقد أظهرت نتائج الـPCR عائديه 5 عزلات لنوع E. faecalis وعزلتين لنوع E. faccium.من خلال دراسة بعض الخصائص المناعية لعزلات Enterococcousبما في ذلك البلعمة والمقاومة لمصل الدم, أظهرت النتائج إن عزلتين من E. faecalis كانت حساسة للبلعمة والمصل، بينما كانت عزلة واحدة من النوع E. faccium مقاومة لكلا الاختبارين المذكورين.} }