TY - JOUR ID - TI - In vitw sperm activation using culture medium supplied with oxytocin in astlienazoospermic patients تنشيط النطف في الزجاج باستخدام الوسط الزرعي المجهز بالاوكيتوسين في مرضى وهن النطف AU - عدنان صالح الجنابي AU - فريال عبد المناف المهداوي AU - مكارم قاسم اللامي PY - 2012 VL - 2 IS - 3 SP - 26 EP - 30 JO - Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم SN - 26166984 22180265 AB - BackgroundPretreatment of the semen samples of the asthenozoospermia patients with certain sperm I stimulant agents prior to use for assisted reproductive techniques (ART) has been reported to I have beneficial effects on fertilization rates.ObjectivesInvestigating the influence of addition of different concentrations of oxytocin (OT) to semen I samples of asthenozoospermic patients for in vitro sperm activation as a new method for I stimulation of sperm motility in these patients and exploring the concentration of OT that I harvests a maximal number of motile sperms.Methods:Simple layer technique for in vitro sperm activation using culture medium enriched with OTwasIstudied in asthenozoospermic patients (No. 90). Semen samples were examined before spermIactivation, and then they were divided into three equal groups. In the first group, threeIconcentrations of OT (2,4, and 6 lU/ml) were applied. Based on the results of the mentionedIwork, OTat the concentration of 2 lU/ml was applied with sperm preparation medium (SPM) andI without in the second and third groups, respectively.ResultsResults showed that there was a significant improvement in the percentage of sperm motility and I sperm gradeactivity of the OT-treated samples atthe concentration of 2 lU/ml ascompared to the I control samples. Moreover, there was a tendency for a decrease in sperm grade activity with I increase OT concentration. The outcome of sperm activation in presence of SPM revealed a I significant increase in the sperm motility characteristics (the percentage of sperm motility and I sperm grade activity) in the control and OT- treated samples and the best improvement in these I sperm motility characteristics was observed in the OT-treated samples. When SPM was absent, I a significant improvement in the sperm motility characteristics was observed only in the OT- I treated samples.ConclusionThe results of this study indicate that OT at the concentration of 2 lU/ml has got a stimulatory I effect on sperm motility characteristics; also, the use of SPM succeeds to support this effect

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