TY - JOUR ID - TI - Occurrence of CTX-M-I Type β-lactamases Gene in Certain Gram Negative Bacteria AU - Ibtesam Ghadban Auda AU - Sana´a Noori Hussein Al-Kakei AU - Saad L. Hamed PY - 2013 VL - 12 IS - 2 SP - 306 EP - 311 JO - Iraqi Postgraduate Medical Journal المجلة العراقية للاختصاصات الطبية SN - 83601608 98932708 AB - BACKGROUND:The CTX-M-type β-lactamases represent a group with a typical extended-spectrum β-lactamase(ESBL)-resistance phenotype. These enzymes, encoded by transferable plasmids. They have apreferential hydrolysis of Cefotaxime over Ceftazidime. The CTX-M-type β-lactamases have beendescribed in species of Enterobacteriaceae and Pseudomonas aeruginosa. OBJECTIVE :This study was designed to investigate of the occurrence of CTX-M-I type in some Gram negativebacteria species isolated from clinical cases of in Iraq. METHODS: A group of Gram negative bacteria were isolated from different sources.Plasmid DNA extraction,and electrophoresis were performed. Using specific primers, CTX-M-I enzyme genes wereamplified by PCR. RESULTS: Plasmid profile of the tested isolates reveals the presence of relatively large plasmids, their Wight was more than 10 kb some isolates posses’ 3-4 kb plasmids. The results of PCR amplificationshowed the presence of CTX-I genes. All isolates of Salmonella enterica serovar Typhimurium(100%) are negative for CTX-M-I gene as well as most of P. aeruginosa isolates (86.7%). Incontrast, all of E. coli (100%) and most of Proteus Spp isolates were positive for CTX-M-I gene.CONCLUSION: CTX-M genes are predominant in E.coli followed by Proteus Spp. while Salmonella entericaserovar Typhimurium and P. aeruginosa isolates showed low incidence of blaCTX-M genesoccurrence. The alarming situation with dissemination of CTX-M producing isolates highlights theneed for their epidemiological monitoring and prudent use of antimicrobial agents

ER -