TY - JOUR ID - TI - The Effect of Three Coating Materials on the Candidal Growth, on the Surface and Color of A heat–Cure Acrylic Resin Denture Base AU - Enas Yaseen Shehab AU - Nadia T Jaffer AU - Monia MN Kandil PY - 2009 VL - 9 IS - 2 SP - 279 EP - 288 JO - Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان SN - 18121217 19980345 AB - Aims: To evaluate the effect of three coating materials: Acrylic resin coating "monopoly", olive oil and
grape seeds oil on the Candida albicans growth, and on color of a heat – cured acrylic resin denture base.
Materials and methods: Three coating materials underwent pores method to find out their antifungal
effect. The effect of coating materials on the acrylic denture base surface was determined via Sensitivity test
in which twenty discs of acrylic resin 6mm diameter and 2mm thickness were prepared and divided into
four groups: control (uncoated), monopoly, olive, and grape oil coated groups. These discs were added to
Sabouraud Dextrose agar surface and incubated for 24 hours at 37 oC and the diameter of growth inhibition
zones were measured. Then the Turbidity test was taken place, in which 60 acrylic specimens (1cm x 1cm x
2mm) were prepared and immersed in 60 test tubes that were divided into four groups, each group (15
specimens) was subdivided into three groups of incubation: 7, 20, and 30 days incubation period, each test
tube contained 4 ml Brain Heart Infusion Broth, 0.1 ml of 24 hours Candida albicans, and its corresponding
acrylic specimen, turbidity (yeast growth) was measured by a spectrophotometer at 530 nm wave length.
For color change measurement test, 20 acrylic resin specimens (45 x 10 x 2.5 mm) were prepared and
distributed into four studied groups. The absorbed light was measured by spectrophotometer and termed
optical density at 345 nm. Results: All coating materials were fungicidal in pores method. Statistical
analysis of sensitivity test showed that Candida albicans was susceptible at highest extent to monopoly
coated denture base followed by the oils coated groups. In turbidity test, monopoly specimens showed
antifungal activity in all periods of incubation, but the effect decreased gradually, olive and grape oil coated
specimens exhibited their highest antifungal activity after 30 days incubation. Color change measurements
showed that monopoly coated group had the highest optical density, while the olive and grape groups
showed a decrease in the optical density when compared to uncoated group. Conclusions: Use of coating
materials on the acrylic denture base surface was beneficial, especially monopoly in terms of antifungal
activity against Candida albicans followed by natural oils (olive and grape oils). However, coating
materials showed a significant change in the color of acrylic denture base.

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