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The current study deals with measuring the level of interferon-ɣ for 96 sample of infected women with Toxoplasma gondii , whose attended Salah- El-Deen Teaching Hospital in Salah- El-Deen Governorate from 13/10 /2016 to20/4/ 2017. The samples have been divided into 50 infected pregnant women with Toxoplasma gondii and 46 women as a control group which included 23 pregnant women and 23 non – pregnant whose as not infected. The study shows significant difference in the level of interferon ɣ between the infected women group and control group .The level of interferon ɣ in infected women was higher compared with the control group 1.658± 0.977 and 0.765 ± 0.524 pg. / ml, respectively, at the level of probability p< 0.01. concerning on to the measurement of the level of the complementary proteins C3, C4, It was shown the result exist variances moral if were valued in infection women of 0.3708± 0.0107 and 0.3851± 0.0306 mg/dl, respectively, compared to the valued in non-infection women 0.2528± 0.0463 and 0.2142±0.0608 mg/ dl, respectively, at the level of probability p< 0.01.

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Abstract: Background: Toxoplasmosis is caused by intracellular protozoan parasite; it is difficult to distinguish between the recently acquired infection and the chronic infection because of the frequent presence of Toxoplasma antibodies in the general population.Objective: This study was conducted to estimate the relationship between the Toxoplasma antibodies titers and other immunoglobulin and complement componentsStudy design: Sera from 40 Iraqi blood donors of different ages (18-45 years) during first of February- first of December /2008 in Baghdad city, were screened for Toxoplasma antibodies titers (28 positive and 12 negative). Agglutination method was used according to the kit procedure (Toxo Cell Latex) and single radial immunodiffusion plates were used for estimation of IgM, IgA, C3 & C4.Results: The results showed a significant differences (P<0.01) in the all means of IgM in all titers compared to controls, and the highest titer of IgM (197±21.27 mg/dl) has been shown at a titer of IgG 160 IU/ml. There was no significant differences (P>0.05) shown between IgA titers compared with IgG titers, while there were a high significant differences (P<0.01) of C3 & C4 compared with the IgG titers at level 10, 20&10 IU/ml respectively. Conclusion: The results indicated that there is a relationship between titers of IgG and titers of IgM and complement components (C3 & C4) during the course of Toxoplasma infection especially at the beginning and chronic infection when the level at IgG titers was low.Key wards: Toxoplasmosis, IgG, IgM, C3, C4

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ABSTRACT:BACKGROUND:lupus nephritis is a fatal complication of systemic lupus erythematosus (SLE), the serological hallmark of SLE is the presence of circulating autoantibodies directed against a wide variety of nuclear, cytoplasmic and plasma membrane antigens among which are anticardiolipin (ACL),anti-B2glycoprotein and anti-C1q antibodies, together with the consumption of complement component (C3 &C4) by immune complex mediated reaction.AIM OF STUDY:To shed light on the relations among these autoantibodies and with complement component in lupus nephritis patients.METHODS:Tthe study was conducted on 25 patients with lupus nephritis, attended the renal clinic in specialized surgical hospital/ medical city /Baghdad, 25 lupus patients without nephritia and 25 healthy controls.Enzyme linked immunsorbant assay was used for detection for ACL, anti-B2GP, anti-C1q, while single radial immundiffusion plates used for the estimation of C3& C4.RESULTS AND CONCLUSION:Anticardiolipin antibodies correlated significantly with anti-B2GP IgG & IgM (P<0.05), anti-C1q antibodies (P

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AbstractThe aim of the present study was to verify the relevance of complement, C3 & C4 with fasting blood glucose level (FBGL) in type 2 obese diabetics. To achieve this aim, 22 obese diabetic patients (12 females & 10 males) with ages of 45.23±13.83 and body mass index (BMI) of 34.52±2.15 Kg.m‾² were enrolled. To compare the results, 20 healthy individuals (10 females & 10 males) of ages 36.55± 5.36 were also included. Serum C3 and C4 levels were measured in the patients & the control group by a single immune-diffusion method. Fasting blood glucose level was estimated by the glucose-oxidase method. In the diabetic patients, serum C3 level was found to be significantly (P<0.01) elevated when compared with those of healthy individuals whereas C4 didn't show such variation. The linear regression analysis exhibited a significant positive correlation for C3 (r= 0.45, P< 0.05) with FBGL in diabetics but not in healthy subjects. Such correlation was also significant (r=0.60, P< 0.001) with the BMI values. In conclusion, C3 was found to direct the levels of FBGL in type 2 obese diabetic patients.

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Background: Measles remains a major cause of worldwide childhood mortality, and has been targeted by the WHO for global eradication following the eradication of poliomyelitis. Despite the implementation of mass school catch-up campaigns for measles, an outbreak of measles occurred mostly affecting the adult population.Objectives: To measurement of Highly Sensitive C Reactive Protein (Hs CRP) and C3 Complement levels in adult volunteers after vaccination with live attenuated measles containing vaccine, and to compare the results with that following vaccination. Subjects and methods: A sero-surveys for serum Hs CRP and C3 complement levels was conducted among [190] healthy young adults aged 18-25 years, randomly selected, and to compare after a national campaign with measles containing vaccine. Hs CRP and C3 complement were detected in volunteer’s sera prior to, 1, and 4 weeks after vaccination, measured by Enzyme Linked Immunosorbent Assay and Single Radial Immunodiffusion Assay respectively. Results: There was a significant differences (p ≤ 0.05) in the concentration of serum Hs CRP and C3 complement at week one following vaccination with measles containing vaccine, and returned to normal after four weeks. Conclusions: Raising Highly Sensitive C Reactive Protein and Complement C 3 Levels in the sera of adult volunteers after measles containing virus vaccine administration could play a role in increasing innate immune response against measles virus infection and may explain some mechanisms of immune response associated with measles infection.

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Background: Gastric ulcer is common and serious diseases caused by imbalances between naturally occurring defensive factors such as mucus, prostaglandins and aggressive factors such as hydrochloric acid, usually found in gastrointestinal juices Objective: To evaluated the level of complement protein C3 and C4 for infected patients with gastric ulcer in Baqubah city, Iraq. Patients and Methods: In this case- control study (57) blood samples were collected from patients with gastric ulcer and (32) blood samples from healthy individuals has been accredited as a control group and the study has continued during the period between October 2016 and April 2017.Results: The results showed that there was a decrease in the level of C3 in patients with gastric ulcers by (25.168 ± 182.173) mg/dl compared to the control group and (501.565 ± 481.418) mg/dl and found that an increase in the level of C4 in patients with gastric ulcer was (34.978 ± 91.252) mg/dl compared to control group and (7.493 ± 17.403) mg/dl in (p value < 0.001). Conclusion: We conclude from this that the complemental protein plays an important role in the localized response to gastric ulcer and gastrointestinal diseases. The presence of neutrophil cells leads to an increase in the production of the C4 complement in patients and that IL-10 has a role in regulating the complemental product in general .

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ABSTRACT:BACKGROUND:Hydatid disease is a widely distributed disease caused by the larval stage of the Cestode Echinococcus. Inflammation increases the synthesis of immunoglobulins and complement components (C3 and C4) presumably through the action of interleukin 1& gamma interferon.OBJECTIVE:Assessment of immunopathotogical response of scrum immnnoglobulins and complement components fC3 and C4)in Iraqi patients that might be a fleeted as inflammatory response to hydatid disease.SUBJECT AND METHOD:A total number of 50 patients with radiological diagnosis of HD wore studied. Surgical interference had been done to 20 out of 50 patients and the results of surgery gave a clinical confirmation of the diagnosis of HD. Sera from patients with HD and 38 apparently healthy controls were obtained for measurement of immunoglobulins (lgG. IgM and IgA) and complement components (C3 and C4) by Radial Immunodiffuision Assay ( Biomaghreb).RESULTS AND DISCUSSION:Hydatid patients showed increases of IgG (P value < 0.001) IgM and IgA levels (p value <0.05) in comparison to the control group, but these variables start to decrease after surgical treatment that is significant only in case of IgM (P value <0.05). and this might be due to activation of humoral immunity against HD. Complement components were found to be insignificantly lower in patients with HD in comparison to control group (P value > 0.05) and higher in one week postoperative period in comparsion preoperative period (P value > 0.05). This might mean consumption of complement and it's activation or it might be due to anticomplementary action of the hydatid cyst.CONCLUSION:The picture that has emerged from this study is that HC wall might have anticomplementary action. And serum complement component tend to increase in postoperative period.

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ABSTRACT: BACKGROUND: To evaluate complement activation (C3) levels in all forms of acute coronary syndrome (ACS) and to find whether there is any significant changes in C3 concentration at the 1st and 4th day after admission and its relation to clinical outcome. OBJECTIVE: Comparing the degree of complement activation (C3 level) between ACS and stable pectoris.To know whether there is any significant difference between the level C3 at first and fourth day. Any correlation between CRP and C3 in patients with ACS. PATIENT AND METHODS: 129 subjects (94 male and 35 female) age range (41-72 years, mean age 57 ± 10.6) were admitted in this study over the period of Feb 2009-Jan 2010 categorized into three groups; 76 patients with acute coronary syndrome (group A), 25 patients with stable angina (group B) and 28 healthy control (group C). Full clinical, biochemical, electrocardiographic and echocardiographic studies liveredone. All patients were followed to the fourth day of admission, Blood samples from peripheral veins were collected centrifuged and Serum C3 levels were measured using immunokit based on single immunodiffusion. RESULTS: The sample of patients was (129) subjects (94 male 72.9%) and (35 female 27.1%). Troponin (I) was positive in 35.7% and negative in 64.3% of the study sample (p. value 0.0005). C-reactive protein (CRP) was significantly correlated with different groups (p. value 0.0004).the same with diabetes mellitus (p. value 0.0003) but not in hypertensive and smokers (p. value 0.486 and 0. 368 respectively).C3levels was significant in correlation to clinical status in both STEMI and NSYEMI 1st and 4th day. Correlation between C3 and C-reactive protein level was insignificant with different groups. CONCLUSION: C3 levels was significantly elevated in correlation between ACS compared to patient with stable angina and healthy control subjects. Also C3 level was significant at the fourth day of admission in patients with NSTEMI in correlation to its level at the first day. However no significance associations between C3 levels and CRP in different studied groups

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ABSTRACT Chronic lymphocytic leukemia (CLL) is a lymphoproliferative disorder characterized by the accumulation of mature-appearing lymphocytes in the blood, marrow, and lymphoid tissues. This study aimed to evaluate innate immunity in patients with CLL via estimation of serum levels of Interleukin-1α (IL-1α) and interleukin 8(IL-8), and complement components C3 and C4. Blood samples were collected from 48 patients with CLL (28 treated and 20 untreated). Other 20 blood samples were collected from healthy appearing individuals as control group. Enzyme linked immunosorbent assay (ELISA) was used to estimate serum concentration of IL-1α and IL-8. Single radial immune-diffusion assay was used to estimate the serum levels of C3 and C4. Total white blood cells and absolute lymphocyte count in each blood sample were determined. The study showed a significant decrease in serum concentration of IL-1α and complement components C3 and C4 in treated and untreated patients, whereas, serum concentration of IL-8 and blood absolute lymphocyte count showed significant increase in untreated patients. These data collectively indicated the aberration in innate immunity in CLL patients which can used as a prognosis for the disease.

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Abstract Objective: To confirm the importance of Chromium trace element in complicated diabetic foot in addition to complement C3 & C4.Patients & Methods: This study was done in Al-Yarmouk Teaching Hospital “Diabetic Center” and Al-Karama Teaching Hospital throughout a period from November 2002 – July 2003. It included 60 diabetic patients who were divided into two groups, depending on the presence of complications. In addition the study included 20 healthy subjects matched for age and sex as control group. Measurement of F.B.S. was done for each patients. Measurement of serum level of Chromium, by using shimadzu atomic absorption flame emission spectrophotometer and measurement of C3 & C4 complement by using immune diffusion assay kite was also done for each subject.Results: Study reveal that there is lowered Chromium trace element and a lowered level of C3 complement than C4 among complicated diabetic foot more than other groups. Conclusion: We concluded that there is a deficiency of Chromium among a complicated diabetic patient in addition to that this study show deficiency of complement C3 more than C4. Key ward: Diabetes mellitus, chromium trace element, complement C3 and C4.

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