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In this research, two activated sludgeexperimental laboratoryplants wereconstructed and operated using dairy wastewater. One of these plants were operatedat high temperatures of (40 – 60 oC) “Thermophilic microorganisms reactors",whereas the other one were operated at constant temperature of (25 oC)“Mesophilicreactor”. The resultsshowed that the organic removal efficiency of "Mesophilicreactor" was more than the "Thermophilic reactors" and it decreasedwith increasingthe temperature.The kinetic parameters for the both mode of operations were alsocalculated. The value of the substrate removal rate constant (k) for the "Mesophilic,25ºC" reactor was (0.017 day-1) and it was greater than others for the thermophilicreactors.(k) and (Y) decreased with increasing the temperature. The values of (Ks),(Umax), and (Kd) in the thermophilic reactors were more than the corresponding valuesin the mesophilic reactor and increased with increasing the temperature.

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Abstarct

The present study was aimed to study the optimum conditions of producing yeast biomass and lytic enzymes, and removal of total solids from dairy waste water. Results showed that dairy waste water serve as a good substrate, enabling the growth of Saccharomyces cerevisia S4, which produced a considerable amount of yeast biomass. Maximum production of biomass was 26g/l obtained after 96h fermentation, at pH6, temperature 30°C and dairy waste concentration of 2.5% w/v. The maximum activity of α-amylase and protease were (67.7, 60.2)U/mg, respectively obtained when cultured the yeast in dairy waste water 2.5%w/v at pH6 and incubated for 120h at 30°C. The maximum reduction of total suspended solids and total dissolved solids were (44.1,53.6)% respectively observed after 96h of fermentation period at 30°C.

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