Abstarct

This study was designed to investigate the cellular changes of peripheral blood lymphocytes (PBL) in children with type 1 diabetes mellitus (T1DM) by determining the surface antigens (CD markers). Sixty T1DM patients who were newly diagnosed as type1diabetetics (duration of their illness is less than five months) were selected from the National Diabetes Center at Al-Mustansiriya University during the period May 2004 to October 2005. All the patients were treated with daily replacement doses of insulin. Their age ranged from (3-17) years. Fifty apparently healthy control subjects, matched with age (4-17) years and sex underwent the PBL phenotyping. Phenotyping of lymphocyte surface antigens was done by direct Immunoflurocent (IF) technique using mouse antihuman CD3, CD4, CD8, CD19 and CD56.T1DM patients showed a remarkable lowering in CD3+, CD8+, and CD56+ cells but the decrease in CD4+ cells percentage was not significant in patients in comparison to healthy controls. In contrast, a significant elevation of CD19+ cell percentage and CD4+: CD8+ ratio were observed in the patients.

Keywords

Export bibliographic record

Choose an application

• Reference Manager
• EndNote
• BibTex

Abstarct

SUMMARY:BACKGROUND:In Type 1 Diabetes Mellitus (T1DM), numerous changes in the cellular as well humoral immune response have been identified. However, it is not known whether both the CD4+ and CD8+ subpopulation or only one of these or CD19+contains increased numbers of activated cells.OBJECTIVE:The aim was to study the activated lymphocyte subpopulation by use of monoclonal antibodies to T-cell and B-cell antigens which is known to be expressed on activated cells.METHODS:A total of 60 T1DM patients who had newly onset of the disease (diagnosed was from one week up to five months) were included in the present study, all the patients were treated with daily replacement doses of insulin. Fifty apparently healthy control subjects underwent the PBL phenotyping. Phenotyping of surface antigens was done by direct Immunoflurocent (IFT) technique using mouse antihuman CD3, CD4, CD8, CD45RA, CD19, and activated markers CD45RO, DR-antigen and CD38.RESULTS:T1DM patients showed a remarkable lowering in CD3+, CD8+, and CD45RA+ cells (p<0.0001), but the decrease in CD4+ cells percentage was not significant. In contrast, a significant elevation of activation markers includes (CD45RO+, HLA-DR+ and CD38+ cells) were observed in patients in addition to a significant increase of CD19+ cell percentage and CD4+: CD8+ ratio in the patients.CONCLUSION:This study provides evidence that abnormalities of T-cells regulation are detectable in patients with T1DM.

Keywords

Export bibliographic record

Choose an application

• Reference Manager
• EndNote
• BibTex

Abstarct

Background: B-cell malignancies are clonal expansions of B-cells that express only one type of Ig light chain (L or k) or (rarely) lack surface Ig light chain expression . Multiparameter color flowcytometry allows analysis of light chain expression in total CD19 + B-cell population and also in various subsets of B cells such as CD5+/CD19+, CD23+/CD5+/CD19+or CD10+/CD19+ positive cells. Objectives: This study was designed to assess the immune phenotyping of 30 patients with chronic lymphoproliferative diseases using flowcytometer.Methods: 30 patients with chronic lymphoproliferative disorders has been investigated by flowcytometry. This study done in Al Rawabie laboratory in period between january2013 till November 2013.Results: Among 30 patients with chronic lymphoproliferative diseases , 18patients diagnosed as chronic lymphocytic leukemia, 3 with follicular lymphoma, 6 with mariginal zone lymphoma, 2 with splenic lymphoma with villous lymphocyte and one case of multiple myeloma. Conclusion: Multiple parameter immunophenotyping analysis improves the accuracy of diagnosis in lymphoid malignancies, and can be used in diagnosis, which easily detects infrequent, hematological malignancies differentiated diagnosis, and detection of minimal residue in lymphoproliferative diseases the simultaneous recognition of different cell populations allows the diagnosis of composite cell lymphomas, or double pathologies.

Keywords

Export bibliographic record

Choose an application

• Reference Manager
• EndNote
• BibTex

Abstarct

This study has been conducted on blood samples from patients with chronic lymphoid leukemia’s (CLL) who werediagnosed and treated at The National Center of Hematology between January 2006 to September 2009.The diagnosis was established depending on clinical and hematological findings. Immunophenotyping was performedby indirect immunofluorescence technique using fluorescent microscopy to identify CD 23 and CD 6 markers expressionon peripheral blood mononuclear cells. Positive cell is seen with multiple fluorescence dyes around the membrane or bybright green membrane fluorescence, the cases considered as positive for marker when the marker is expressed in ≤ 30of cells.Sixty cases were newly diagnosed untreated CLL patients with mean age of 64.5 ± 10.1 years with 9.4% of casesbelow the age of 45 years and male to female ration of 3:1were involved in this study.Immunological study showed that all the cases were CD 23 positive while CD6 were positive in 45 (75%) out of 60cases and negative in 15(25%) out of 60 cases. These percentages in all CD 23 positive cases and CD6 positive CLLpatients were higher significant than normal group (p=0.000).In conclusion, the positive CD23 expression clarify that the majority of chronic lymphoid leukemia are B-cell type.B-cell is the most common which is heterogeneous disease regarding clinical presentation ,hematological findings andmorphological feature although CD6 is not specific marker for diagnosis of CLL ,but it may help in the diagnosis of CLL.

Keywords

Export bibliographic record

Choose an application

• Reference Manager
• EndNote
• BibTex

Abstarct

ABSTRACT:BACKGROUND:Immunophenotyping with monoclonal antibodies (MoAbs) directed against lymphoid-associated antigens, immunohistochemical staining on paraffin-embedded BM biopsy material, and molecular studies of Ig genes/T-cell receptor genes or lymphoma-associated gene translocations should be used in the global approach to the patient with malignant lymphoma.OBJECTIVE:1.To determine the subtypes of non-Hodgkin lymphomas (B- or T cell) in the bone marrow using anti-CD3, CD8 monoclonal antibodies for T-cell and anti-CD19 and CD20 for B-cells. 2.Correlation of the subtypes of NHL (B- or T cell) with the morphology and pattern of bone marrow infiltration. PATIENTS, MATERIALS AND METHODS:A retrospective study, done in Al-Kadhymia teaching hospital during the period from 1/10/2010 to1/2/2011.The study consisted 26 adult patients, who were diagnosed as Non-Hodgkin lymphomas by undergoing a BM biopsy. Immunohistochemical staining of the paraffin-embedded sections of BM trephine biopsies was performed in all cases and used standard techniques with monoclonal anti-CD8, CD20 and dual immunofluorscence-labelled CD3, and CD19 antibodies and also all stained with Hematoxylin and Eosin (H&E) for morphologic assessment.RESULTS:The 26 cases of NHL comprised of 14 male (54%) and 12 female patients (46%) . The median age was (57.32) year ranged from 27-85 years. There were 23 cases of B-cell cases (88.5%) and 3 cases of T-cell lineage (11.5%) of all the cases. Among all the B-cell lymphomas, 15 cases showed interstitial infiltration in the bone marrow, while among the T-cell lymphoma two cases showed diffuse infiltration. CONCLUSION:1.In these 26 cases NHL patients with marrow involvement, B cell phenotype comprised 88% of cases.2.B-cell NHLs had predominance of interstitial infiltration in bone marrow biopsies in comparison with the T-cell lymphoma, in which diffuse infiltration was predominant..

Keywords

Export bibliographic record

Choose an application

• Reference Manager
• EndNote
• BibTex

Abstarct

ABSTRACTBackground: Immunophenotyping has become crucial in the diagnosis and classification ofacute leukemias and identification of its aberrant phenotypes. This study aimed to evaluatethe patterns in AML cases presented to the flowcytometry unit at the Sulaimani Public HealthLaboratory, Kurdistan, Iraq,and to determine the frequency of aberrant expression oflymphoid associated antigen.Subject and Methods: For the above purposes, 108 cases of acute myeloid leukemia (AML)were evaluated morphologically,and by flowcytometry with a panel of 22 antibodies.Furthermore, any aberrant expression of lymphoid associated antigens was reported.Results: The morphological AML subtyping revealed that 29.6% of AML cases were M2,while M1 (36.1%) was the predominant subtype by immunophenotyping using WHO 2008classification. CD117, CD45, CD13 and CD33 were the most frequently expressed markers(99.1%, 92.6%, 92.6% and 85.2% respectively). Forty-five patients (41.7%) expressedlymphoid associated antigens (nTdT, CD19, CD79a, CD10, CD4, CD56 and CD9) that wasdemonstrated in all AML subtypesexcept M6.CD56 was the most frequent(13%),followedby CD9 (12%), CD4 (8.3%), and CD19 (7.4%).CD79a, nTdT and CD10 were less frequent,present in <5% of cases each.Conclusions: Immunophenotyping is an essential supplement to morphology in AML,whether for confirmation or accurate subtyping. Furthermore, more than 40% of cases showaberrant lymphoid antigen expression. The latter may serve as an important tool in futurestudies on minimal residual disease evaluation.

Keywords

Export bibliographic record

Choose an application

• Reference Manager
• EndNote
• BibTex

Abstarct

BACKGROUND: Splenic marginal zone lymphoma (SMZL) is a low‑grade disorder that regularlypresents with peripheral blood (PB) involvement. A precise description of clinical, laboratoryfeatures and immunophenotypic characterization of SMZL are still lacking. Here, we reviewed34 patients presenting with SMZL to describe the clinical, hematologic features, and flow cytometryimmunophenotypic findings of this type of lymphoma at diagnosis.OBJECTIVES: The aim of this study is to confirm that SMZL has a specific immunologic profile whichenables the hematopathologist and clinician to differentiate this low‑grade B‑cell lymphoma from otherB‑cell lymphoproliferative disorder, especially chronic lymphocytic leukemia and hairy cell leukemia,which could sometimes stimulate SMZL morphologically and to emphasize that a correlation ofimmunophenotypic findings, clinical, and hematologic features of patients plus careful morphologicalexamination of PB and/or bone marrow (BM) aspirate can lead confidently to the correct diagnosis.MATERIALS AND METHODS: Flow cytometry immunophenotypic findings of 34 cases of SMZL werereviewed. The analysis was performed by BD FACS Calibur™ and FACSCanto II flow cytometers.B lymphocytes were identified according to their Side-Scattered (SSC)/CD19 distribution. A markerwas considered positive when expressed in more than 20% of cells above the control.RESULTS: Median age was 60 years, range (35–84 years), both sexes were affected equally. Allpatients presented with splenomegaly, 71% of patients had absolute lymphocytosis and 88% ofpatients showed PB involvement. Seventy‑four percent of patients had anemia and (53%) of themhad thrombocytopenia. Cells from all cases expressed pan B‑cell antigens (CD19, CD20), 74% ofcases expressed CD79b and Human Leukocyte Antigen – antigen D Related (HLA-DR) expressedin nearly almost all cases (97%). Half of the patients expressed CD11c and SIgD, 41% expressedCD5 and FMC7 while CD25 and CD103 showed positivity in less than 5% of cases. Preferentialexpression of Kappa light chain was demonstrated, CD10 and CD38, SIgG were negative.CONCLUSION: SMZL has a distinct immunologic profile which if correlated with morphologic findingsof PB or BM aspirates, clinical and hematologic features can help to make the accurate diagnosisand lessens the need of further invasive diagnostic procedure.

Keywords

Export bibliographic record

Choose an application

• Reference Manager
• EndNote
• BibTex

Abstarct

BACKGROUND: Immunophenotypic analysis of leukemic cells by multicolor flow cytometry usingdifferent monoclonal antibodies labeled to various flourochromes, is an important, precise and rapidinvestigation for diagnosis, classification, prognosis prediction, and minimal residual disease detectionin acute lymphoblastic leukemia (ALL).OBJECTIVES: The aims of study were to study the expression of CD45, CD34, CD10, humanleukocyte antigen‑DR (HLA‑DR) in B and T‑ALL among 114 Iraqi patients and compare findingswith other reports worldwide.PATIENTS, MATERIALS AND METHODS: A retrospective cross‑sectional study was conductedon 114 ALL patients of various age groups from different hematology centers in Baghdad who werereferred to Flowcytometry Department at the Bone Marrow Transplantation Center/Private NursingHome Hospital/Medical City for immunophenotypic classification using multi‑color flow cytometryfrom the January 1, 2016, to the August 31, 2016.RESULTS: Out of 114 patients, 71 patients were pediatric patients and 43 were adults. Eighty‑threepatients were classified as B‑cell ALL and 31 patients as T‑cell ALL. There was significant associationbetween male gender, high white blood cells count and T‑cell ALL subtype. Negative‑dim‑moderateCD45, positive CD34, and positive HLA‑DR expressions were significantly associated with B‑cellALL. Common ALL antigen was seen in 86.7% of the B‑cell ALL patients and was accompaniedcTdT expression. Aberrant myeloid antigens were observed in 22.9% of B‑cell ALL patients and in35.5% of T‑cell ALL. About 4.8% of the patients with B‑cell ALL had aberrant T‑linage antigens while6.5% of the patients with T‑cell ALL expressed aberrant B‑cell lineage antigens.CONCLUSION: Immunophenotypic expression of ALL cells among Iraqi patients is to somewhat inaccordance with various researches worldwide making immunophenotyping flow cytometry a crucialappliance in diagnosis, classification, risk stratification, and minimal residual disease detection in ALL

Keywords

Export bibliographic record

Choose an application

• Reference Manager
• EndNote
• BibTex

Abstarct

Background: In Type 1 Diabetes Mellitus (T1DM), numerous changes in the cellular as well humoral immune response have been identified. However, it is not known whether both the CD4+ and CD8+ subpopulation or onlyone of these or CD19+ contains increased numbers of activated cells.Objective: The aim was investigate the expression of CD38 as activated marker on the peripheral blood lymphocytes of T1DM children at the onset of the disease.Patients and methods: A total of 60 T1DM patients who were newly onset of the disease (diagnosed less than five months) were included in the present study, all the patients were treated with daily replacement doses of insulin. Fifty apparently healthy control subjects underwent the peripheral blood lymphocytes (PBL)phenotyping. Phenotyping of surface antigens was done by direct Immunoflurocent (IF)technique using mouse antihuman CD38.Results: Increased percentage of activation marker CD38+ cells were observed in T1DM patients (24.72%, 23.83%) as compared with the control group (16.86%, 15.97%) in the age group ≤10 years and >10 years old respectively. These differences were highly significant (P1=0.0001)between the patients and healthy individuals, but failed to reach a significant level (P2= 0.44)between the patients in both age groups.Conclusion: A significant elevated percentage of CD38+ activation marker cells were detected in the patients.

Keywords

Export bibliographic record

Choose an application

• Reference Manager
• EndNote
• BibTex

Abstarct

Objective: To assess the relationship between the serum concentrations of vitamins C & E and the severity of coronary artery disease.Subjects and methods: In a case-control study, we evaluated 200 patients who underwent coronary angiography at AL-Basrah Cardiac Center at Al-Sader Teaching Hospital, Basra, Iraq. They were separated into two groups of case (patients with CAD) and control (non CAD). Four milliliters of blood samples were taken for measuring vitamin E and C. For statistical analyses, chi-square test, Student’s t-test, one-way ANOVA, and the logistic regression were used.Results: In the present study, 94 participants with CAD in the case group and 106 participants free of CAD in the control group were included in the analysis. At baseline, there were significant differences in serum vitamin C ,vitamin E and some cardiovascular risk factors(diabetes, hypertension and smoking habits) between the two groups (P < 0.05). Moreover, when other risk factors of CVD were included in the model, serum vitamin C (Odd Ratio (OR = 0.8, 95% CI= 0.68-0.92, P = 0.0001) and serum Vitamin E (OR = 0.66, 95% CI = 0.578-0.754, P = 0.0001) were associated with CAD. There were a significant (P < 0.05) statistical changes in the vitamin C and E among the three sub groups of CAD patients being more deficient as the disease become more severe. Conclusions: Low serum vitamin C and E concentrations were associated with CAD and related to its severity.

Keywords

Export bibliographic record

Choose an application

• Reference Manager
• EndNote
• BibTex