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The possible action of high dose of pomegranate (Punica granatum L.) powder extract was evaluated innormal healthy and dacarbazine-induced toxicity in albino rats. Twenty-eight male albino rats were usedin this study. The animals were divided into three different groups in addition to control group. The first grouptreated with intravenous injection of (1mg/kg b.w.) dacarbazine. he rats of the second group supplemented withpomegranate juice with concentration (1000mg/l) in drinking water, while the third group was the combinationof the two treatments. Samples of blood were collected after a weeks of treating, and the serum was obtainedin the laboratory for the evaluation of P53 protein within the serum. The results showed a significant reductionof P53 level in dacarbazine treated rats comparing with control. Whereas, pomegranate juice treated rats hadsignificant increasing of P53 level as compared to control. The level of the combination treatment also wassignificantly more than control and dacarbazine treatment, while it was significantly lesser than pomegranatejuice treated rats. These finding suggests that dacarbazine cytotoxicity is not P53 dependent and high dose ofpomegranate has role in P53 protein expression induction.

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This study was conducted to evaluate the activity of pomegranate (Punica granatum L.) peel powder and Pseudomonas fluorescens against Pythium aphanidermatum the causal agent of cucumber seedlings damping-off. Results showed that augmenting of pomegranate peel powder at 2%, P. fluorescens suspension contain 4×107cfu/ml, and the fungicide Previcure N at 0.5 ml/L to culture media (PDA) were inhibited the pathogen growth by 100, 100 and 72.1% respectively. Results of greenhouse experiment indicated that treatment of cucumber seeds with pomegranate peel powder, reduced pre-emergence damping-off percentage to 3.3% compared with 16.6% disease with P. fluorescens and 96.6% in control treatment (pathogen only). No significant differences were observed between fungicide and pomegranate peel powder (p=0.05), while the fungicide was more active than the bacteria in reducing pre-emergence damping-off percentage. Pre-emergence and post-emergence damping-off of seedlings reduced by both pomegranate peel powder and the bacteria compared with control (pathogen only). P.fluorescens increased fresh and dry weights and plant heights, while pomegranate peel powder didn’t affect these growth parameters.

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Background and objective: Prevention of bacterial adhesion is an attractive target for the development of new therapies in the prevention of bacterial infection. The aim of this study is to investigate the effects of pomegranate peel extract, vitamin C, combination of pomegranate peel extract and vitamin C & gemifloxacin on adhesion of E.coli to uroepithelial cells. Methods: Uroepithelial cells were incubated with E. coli ATCC 25922 bacteria previously exposed to either the aqueous extract of pomegranate peel (AEPP), vitamin C, combination of both or gemifloxacin and the adherence was assessed by light microscopy.Results: AEPP showed good antibacterial activity with minimum inhibitory concentration MIC of 25μg/ml and 10 mmol/ml for vitamin C and upon combination, the MIC was10µg/ml &5mmol/ml for AEPP & vitamin C respectively whereas for gemifloxacin was 0.03μg/ml. In vitro and in vivo adhesion of E.coli to uroepithelial cells were significantly inhibited by AEPP, vitamin C alone and in combination and by gemifloxacin.Conclusion: AEPP showed good antibacterial activity, inhibited E.coli adhesion to uroepithelial cells and were potentiated by vitamin C.

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Background: Pomegranate (punica granatum) fruits are widely consumed as juice. The potent antioxidant and anti-atherosclerotic activities of pomegranate are attributed to its polyphenols including in it. Aim: Pomegranate extract were evaluated for in vitro antiproliferative, cytotoxicity and apoptosis activities against Hep2 (Human larynx epidermal carcinoma) and Hela (cervical human carcinoma) cell lines. Methods: aqueous extraction of pomegranate was prepared and in vitro cytotoxicity was evaluated on two cancer cell lines Hep2 (Human larynx epidermal carcinoma) and Hela (cervical human carcinoma) cell lines.Results: the study showed that the pomegranate extract has greatest antiproliferative activity against the two cell lines used by inhibition proliferation from 13% to 73.9%. At 10 µg/ml, the extract showed inhibitory effect on Hep-2 cell line at 24 hr period of exposure gives 73.9% while the inhibitory effect on Hela cell line at 24 hr was 57.7%. Conclusions: we concluded that pomegranate extract showed an in vitro antiproliferative activity, indicating its potential usefulness to treat different cancer types.Key words: pomegranate extract, cytotoxicity, cancer cell lines.

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This research included isolation of 18 bacterial isolates from children stool with diarrhea and acute bloody water and under the age of five, if possible get a 9 isolates of the bacterium Escherichia coli and 5 isolates of the bacterium Salmonella typhimurium and 4 isolation of the bacterium Shigella flexneri, subjected all of these isolates tests AGRO and microscopic and biochemical and diagnosed using Vitek2 system. Tested the effectiveness of inhibitory extract alcohol to peel pomegranate Punica granatum L. in the growth of isolates bacterial intestinal and using the diffusion method in drilling Well diffusion as well as determine the focus damper Near Minimum inhibitory concentration MIC and focus killer Near Minimal bactericidal concentration MBC extract against these isolates bacterial, showed alcoholic extract of the peel pomegranate a clear impact on all isolates bacterial intestinal and this effect compared with the effect of 10 antibiotics included (Rifampicin, Amoxicilln, Ampicillin, Ciprofloxacin, Trimethoprim, Cefotaxim, Ceftriaxon, Neumycin, Tetracyclin, Chloramphenicol). Inhibitory effect of the alcoholic extract of peel pomegranate has been getting increasing with the concentration of extract, as the focus value of damper minimum MIC and focus killer Near MBC extract against all of the bacteria Escherichia coli and bacteria Shigella flexneri were 1 and 2 mg / ml, respectively, while the damper minimum focus MIC extract value against bacteria Salmonella typhimurium is 2 mg / ml while the killer is not specified minimum value of MBC focus extract against these bacteria.

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Experimental investigations had been done in this study to demonstrate the effect of natural particles used as a reinforcement material to unsaturated polyester resin. The tensile test and water absorption were investigated according to (ASTM D638) and (ASTM D570), respectively. The influence of sunflower husk and pomegranate husk particles, used as a reinforcement material, on the tensile strength, Young's modulus and water absorption with different weight fraction (3%, 7% and 10%) and particle grain size (50µm, 100 µm and 150 µm), has been investigated. The water absorption of polymer composites was studied by measuring the specimen weight before and after immersion in water for one hundred days. In the experiments of tensile test, all specimens loading was performed with (50KN) operating at a crosshead speed of 10 mm/min. It is observed that the addition of sunflower husk up to 10% and pomegranate husk particles up to 7% as reinforcement materials to polyester resin, leads to increase the tensile strength and Young's modulus of the composite material prepared and the use of sunflower husk as a reinforcement material increased the tensile strength, Young's modulus and water absorption were better than pomegranate husk at the same percentage of addition. The decrease in reinforcement material grain size led to increase the tensile strength, Young's modulus and water absorption. Therefore, all the best result seen in composites containing reinforcement material with (50µm). Finally, the best result obtained in tensile strength, Young's modulus and water absorption were with the addition of 10% sunflower husk as a reinforcement material to polyester resin.

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Pomegranate shells were investigated as green inhibitors for steel 44-2 in 0.1M HCl at various temperatures (30, 40, 50, and 60) °C using electrochemical technique. Five concentrations of inhibitor were added 4, 8, 12, and 16 ppm. Corrosion tests were performed by Potentiostat at 5 mV.sec-1 scan rate and the data measured by Tafel extrapolation method such as Tafel slopes, corrosion current density and corrosion potential. Inhibition efficiencies were calculated and indicated that 8 ppm was the best concentration for inhibition especially at 60oC, where was 96.47%. Pomegranate Shells behaves as anodic inhibitor type and obeys Langmuir adsorption isotherm. The equilibrium constant of the adsorption-desorption process and the apparent free energy of adsorption confirm the physicsorption of Pomegranate shells. FTIR spectroscopy was used to test film formed on steel surface compared with FTIR spectrum of Pomegranate Shells. These spectra confirm the formation of Fe2+Pomegranate shells complex.

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Background: Cisplatin is a potent anti-cancer agent used successfully in treatment of cancers of solid organs, but it has a high rate of nephrotoxicity.Objective: The present study was designed to study Cisplatin-induced nephrotoxicity and the nephroprotective property of pomegranate juice.Materials and Methods: The experiment was performed on 36 Iraqi white male domestic rabbits. Rabbits were divided into three groups; control group (received neither pomegranate juice, no Cisplatin), Cisplatin group (received Cisplatin only), and pomegranate group (received pomegranate juice and Cisplatin).Results: Cisplatin group showed marked reduction of renal function manifested by high levels of blood urea, serum creatinine, and low level of serum albumin.Raised levels of oxidative stress markers and severe renal parenchymal damage by histopathology.While, pomegranate group showed almost normal renal function tests and normal levels of oxidative stress markers, and normal renal parenchymal histopathology.Conclusion: Cisplatin in a highly nephrotoxic drug, and Pomegranate juice has a nephroprotective activity against Cisplatin-induced nephrotoxicity.Keywords: Cisplatin, nephrotoxicity, Pomegranate.

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Background: Pomegranate (Punica granatum) fruits are widely consumed as juice. The potent antioxidant and antiatheroscleroticactivities of pomegranate are attributed to its polyphenols.Aim: Pomegranate extract were evaluated for in vitro cytotoxic and its effect on the level of p53 in Hep2 (Human larynxepidermal carcinoma).Methods: Aqueous extraction of pomegranate was prepared and in vitro cytotoxicity and amount of p53 was evaluatedon cancer cell line Hep2 (Human larynx epidermal carcinoma).Results: The study showed that the pomegranate extract has greatest antiproliferative activity by inhibition proliferationfrom 13% to 73.9%, at 10 μg/ml and 24 hr period of exposure. Also, the study showed significant decrease of p53 intreated group in comparison to control.Conclusions: According to the in vitro antiproliferative activity, inhibition of p53 expression pomegramate extractsmay be possess a potent anticancer activity.

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The objective of this study was to investigate the effect of using PomegranatePeel extract on spent hen meat on some physical and chemical characteristics. Theminced hen meat was immersed separately in 0, 0.5, 1and 1.5% ofthe Pomegranate Peel extract. Meat samples were stored individually for 0.3 and 6week at -18 C°. The results showed the immersion of minced spent hen withPomegranate Peel extract a significant decrease (P<0.05) in water holdingcapacity, pH, metmyoglobin pigment, thiobarbituric acid (TBA) and a Simple decrease in cooking loss , drip loss. Also a significant increase (P<0.05) inconcentration of myoglobin.It can be concluded that immersion of minced spent hen meat in PomegranatePeel extract improve the quality of meat when stored under frozen -180C up to 6weeks.

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