Molecular Analysis of “E” Genotyping to Giardiasis In Ruminants In Karbala Province

Abstract

The current study included examination of 540 fecal samples of animals (180 cattle, 180 sheep and 180 goats) from the slaughter house at Karbala province. The animals suffered from diarrhea within the period from October 2013 to the end of June 2014, The current study showed the rate of total infection of Giardiasis was (277 of 540 samples). They were examined by smear method and using light microscope trophozoites and cyst phases of parasite. Molecular results showed in finding three assemblage genotype (A, B, E) and found mixed infection with genotype A & B in (cattle, sheep and goat), there is no significant difference at (P ≤ 0.05) to infection by assemblage genotype E. while the assemblage A there is found significant difference foundation.The foundation of genotype B no significant difference between three animals. Foundation of assemblage A & B together have significant difference between goats and cattle & sheep at P ≤ 0.05) while in cattle we found significant difference with genotype (E, A, B, A & B).In sheep and goats they have significant difference between the three assemblage genotype (E, A, B) and A & B together according to the infection by Giardiasis in animals in Karbala.