Effect of pesticide Glyphosate Aqua in blood picture of Barbus sharpeyi

Abstract

The present study designed in fish diseases laboratory, College of Veterinary Medicine/Baghdad University for a period between 1/3 to 1/6/2013 to investigate the toxicity of the herbicide glyphosate aqua on Barbus sharpeyi fish. Fish ranged between 10-15g. in weight were used to measure the (LC50), fingerlings were used to determine the acute and chronic toxic effect for the herbicide. The fingerlings were randomly distributed as 10 fish for each aquarium. Fish were divided into four treated and control group (without addition of herbicide). The first and second treated groups were exposed to 0.415mg/l of the herbicide for 90 and 15 days respectively, while fishes in the third and fourth treated groups were exposed to 0.207mg/l of the herbicide for 90 days and 15 days respectively. The present study aims to determine the effect of the pesticide on the number of red blood, white blood, hemoglobin concentration and the ratio of the number of packed cells volume, as well as to identify the small number of nuclei red blood cells in the experimental fish. The results of blood picture for the experimental fish showed that there are significant differences (P˂0.05) in the first and third treatments compared with the control group. The first treatment showed a significant decrease (P˂0.05) in the number of red blood cells and hemoglobin, and the percentage of the size of the compacted blood cells as well as the sharp rise in the number of white blood cells compared to the rest of the treatments and the control group. The results of the experiment reveal an improvement in the health status of the second and fourth treatments. In conclusion of this study the greater the concentration of pesticide Glyphosate Aqua, it would have a negative impact on the blood parameters for fish, and could improve the health status of the Bunni when improving environmental conditions for fish and removes exposure to a pesticide