A Study of sensitive S. aureus, E.coli and Ps. aeruginosa for chlorhexidin and sodium hypochlorite in Ramadi Teaching Hospital

Abstract

420 Samples were collected from some environmental locations in Ramadi Teaching Hospital included Floors, Walls, Air Windows, Doors and Sinks, 41 Isolates for S. aureus, 24 Isolates for E.coli and 22 Isolates for Ps. aeruginosa were diagnosed and Sensitive of 12 isolates for these bacteria toward Chlorhexidine and Sodium Hypochlorite were tested through determination MICs and MBCs and killing Times by used Broth Dilution Method, Chlorhexidine were high efficacy from Sodium Hypochlorite toward S. aureus E. coli and Ps. aeruginosa, MICs were range between 4-256 µg/ml for Chlorhexidine and 128-3000 µg/ml for Sodium Hypochlorite, MBCs were range between 8-512 µg/ml for Chlorhexidine and 256-4000 µg/ml for Sodium Hypochlorite and Killing Time for three isolates after exposed to MBCs were range between 10-15 min for Chlorhexidine and between 20-35 for Sodium Hypochlorite, In the finally S.aureus bacteria was the high sensitively for both disinfectants followed by E. coli but Ps. aeruginosa was the high resistance for both disinfectants