Novel method for visualizing of the coated microbubbles using the electron microscopy


The microbubbles have many important acoustics applications, some of these applications are in medical uses such as ultrasound contrast agents and drug delivery. The efficiency and stability of the microbubbles are depending on many factors one of them is the structure of their shells. There is a significant need to visualize this structure to understand and optimize the bubbles behavior. Direct observation such scanning electron microscopy (SEM) and transmission electron microscopy (TEM) pose a big technical challenge because of the vacuum environment required by these devices. In addition, the freeze-fracture technique with TEM is long and expensive. In the present study, a novel modified method was used to overcome these challenges and consider a simple,