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The Association of Methylenetetrahydrofolate Reductase (MTHFR)/C677T Polymorphisms with The Development of Peripheral Neuropathy in Type 2 Diabetes Mellitus

Nawrass Jassim Al-Salihi --- Ihsan Mohammed Ajeena --- Thekra AB Al-Kashwan --- Zuhair Al-Lebban

Medical Journal of Babylon مجلة بابل الطبية
ISSN: 1812156X 23126760 Year: 2016 Volume: 13 Issue: 2 Pages: 452 -458
Publisher: Babylon University جامعة بابل

Abstract

One of important microvascular complication of patients with T2DM is neuropathy, commonly diabetic peripheral neuropathy (DPN). MTHFE/C677T polymorphisms affect MTHFR enzyme activity with subsequent elevation in the toxic homocysteine concentrations that result in vasculopathy and nerve ischemia. This case control study was planned to estimate the association between the MTHFR/C677T polymorphism and the occurrence of peripheral neuropathy in type 2 diabetic (T2DM) patients in a group of Iraqi peopleEighty three Iraqi subjects were included in this study, 36 had diabetic peripheral neuropathy, 25 without peripheral neuropathy and 22 were healthy control.Genomic DNA was isolated from fresh whole blood and genotyped using the polymerase chain reaction–based restriction fragment length polymorphism assay for the MTHFR gene C677T mutation.The distribution of MTHFR/ C677T mutated genotypes between DPN and healthy groups was statistically differed with increased risk of occurrence of DPN (OR= 3.1, 95% CI: 1.02-9.4, p=0.05), while this risk was highly increased by seven folds when comparing DPN group with those without DPN& 2 (OR= 6.7, CI=2.1-20.8, p=0.0008).MTHFR/C677T polymorphism is highly associated with development of peripheral neuropathy in T2DM Iraqi population

Keywords

diabetes mellitus --- diabetic peripheral neuropathy --- methylenetetrahydrofolate reductase --- MTHFR.