Significant of conventional serological tests in diagnostic of Brucellosis in AL-Diwaniyah province

Abstract

The study was conducted on 351 serum samples from suspected cases brucellosisof human (125 ) (68 male and 57 female )of age distribution from (10-70) years and100 sheep (84 females and 16 males) , goat (23 female and 17 male ) and cow (64female and 22 male ) of different ages (1->3 years) suspected selected from October2011 to August 2012 in Diwaniyah province. The Rose Bengal test, tube agglutinationtest and 2- mercaptoethanol test were used to determine the incidence of brucellosis.The highest infection rate of disease was recorded by Rose Bengal test 19.2%, 21% ,17.39% and 8.13% in human sheep ,goat and cow respectively . In human the highincidence was in male where as in livestock animal the highest incidence was infemales in all serological tests and the highest incidence was in females at the agebetween 1-3 years whereas in males more than 3 years of age .The results of tubeagglutination test revealed the titer 1/320 occurred mostly compared with other titers.Eight chronic cases were determined by 2-mercapto-ethanol test in human and 4 ,1and 2 chronic cases in sheep , goat and cow respectively. The degree of agreement ofnegative samples with Rose Bengal test and tube agglutination, and 2-mercaptoethanol tests was 99%, 99% in human and 98.43% and 98.43% in sheep, aswell as 100% in both test for goat and cow respectively .