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The Qualification and Quantification of Caffeine in Two Different Caffeinated Pharmaceutical Formulas Employing RP-HPLC Technique.

القياس النوعي و الكمي للكافيين في مستحضرين صيدلانيين مختلفين محتويين على الكافيين بتوظيف تقنية الكروماتوغرافيا السائلة عالية الاداء-الطور العكوس.

Zaid Al-Obaidi زيد مهدي جابر العبيدي

Albahir journal مجلة الباهر
ISSN: 23125721 Year: 2015 Volume: 2 Issue: 3,4 Pages: 76-89
Publisher: AL-Abbas Holy Shrine العتبة العباسية المقدسة

Abstract

Abstract Background: Method optimization of drug analysis is an arising approach among multidisciplinary science fields. The High performance liquid chromatography (HPLC) has been approved to be the cornerstone of drug analysis. Over the last ten years, HPLC has been figured as the analysis method of choice for many compounds. HPLC system has been utilized in diversified applications such as: the quantitative/qualitative analyses of biological samples, calculating the amounts of active pharmaceutical ingredient (API), isolating specific components from their mixtures, and many others. This work aims to qualify and quantify the caffeine in two different pharmaceutical formulas namely; Panadol® Extra tablets and Thicker Fuller Hair® shampoo employing RP-HPLC. Methods: Isocratic method was utilized with fixed mobile phase ratio as methanol: water (40:60). The flow rate was 0.8 ml/min while the wavelength was 273nm. Calibration curve was plotted via the utilization of the following concentrations (0.0, 20, 40, 60, and 80) µg/ml. Thirty six caffeinated tablet samples were examined as dissolved by methanol and filtered with 0.22 µm nylon-membrane syringe filter and diluted. On the other hand three independent caffeinated shampoo samples were examined. The samples were diluted, shacked, and filtered with 0.22 µm nylon-membrane syringe filters. Finally, the diluted solutions were injected to the RP-HPLC and the data was observed and recorded. Results: The areas required to plot the calibration curve were observed. The caffeine peak was identified within each pharmaceutical sample. Consequently the identified peaks were also quantified. The mean content of caffeine in caffeinated tablets was found to be 64.96 mg. Moreover, the caffeine concentration in the shampoo was observed as 0.0047%. Discussion and conclusion: In this work the RP-HPLC has been utilized in the qualification and quantification of caffeine in two different pharmaceutical formulas namely; Panadol® Extra tablets and Thicker Fuller Hair® shampoo. HPLC technology has matured to the extent that almost any existing drug can be analyzed by an existing method that can be found in the analytical literatures. For future work other drugs can be examined or even multidrug formulas can be tried with such accurate and precise RP-HPLC technique.

ﺍﳋﻼﺻﺔ ﺧﻠﻔﻴﺔ: ﺗﻜﺎﻣﻞ ﻃﺮﻳﻘﺔ ﻟﻠﺘﺤﻠﻴﻞ ﺍﻟﺪﻭﺍﺋﻲ ﻫﻮﳖﺞ ﻣﺘﺼﺎﻋﺪ ﺑﲔ ﳎﺎﻻﺕ ﺍﻟﻌﻠﻮﻡ ﻣﺘﻌﺪﺩﺓ ﺍﻟﺘﺨﺼﺼﺎﺕ. ﺍﻥ ﺗﻘﻨﻴﺔ ﻗﺪ ﺍﺛﺒﺘﺖ ﻟﺘﻜﻮﻥ ﺣﺠﺮ ﺍﻟﺰﺍﻭﻳﺔ ﰲ ﺍﻟﺘﺤﻠﻴﻞ ﺍﻟﺪﻭﺍﺋﻲ. ﺧﻼﻝ ﺍﻟﻌﻘﺪ (HPLC)ﺍﻟﻜﺮﻭﻣﺎﺗﻮﻏﺮﺍﻓﻴﺎ ﺍﻟﺴﺎﺋﻠﺔ ﻋﺎﻟﻴﺔ ﺍﻷﺩﺍﺀ ﺗﻢ ﺍﺳﺘﺨﺪﺍﻣﻪ (HPLC) ﺍﻟﻄﺮﻳﻘﺔ ﺍﻟﺘﺤﻠﻴﻠﻴﺔ ﺍﳌﺨﺘﺎﺭﺓ ﻟﻠﻌﺪﻳﺪ ﻣﻦ ﺍﳌﺮﻛﺒﺎﺕ. ﺍﻥ ﻧﻈﺎﻡ (HPLC)ﺍﳌﻨﴫﻡ ﺗﻘﺮﺭ ﺍﻥ ﻳﻜﻮﻥ ﰲ ﺗﻄﺒﻴﻘﺎﺕ ﻣﺘﺸﻌﺒﺔ ﻣﻨﻬﺎ: ﺍﻟﺘﺤﻠﻴﻼﺕ ﺍﻟﻜﻤﻴﺔ /ﺍﻟﻨﻮﻋﻴﺔ ﻟﻠﻌﻴﻨﺎﺕ ﺍﻟﺒﺎﻳﻠﻮﺟﻴﺔ، ﺍﳊﺴﺎﺏ ﺍﻟﻜﻤﻲ ﻟﻠﻤﺤﺘﻮ￯ ﺍﻟﺼﻴﺪﻻﲏ ﺍﻟﻔﻌﺎﻝ، ﻋﺰﻝ ﺍﳌﺮﻛﺒﺎﺕ ﻣﻦ ﳐﺎﻟﻴﻄﻬﺎ، ﻭﺍﺳﺘﺨﺪﺍﻣﺎﺕ ﺍﺧﺮ￯ ﻋﺪﻳﺪﺓ. ﻫﺬﺍ ﺍﻟﻌﻤﻞ ﳞﺪﻑ ﻟﻠﻘﻴﺎﺱ ﺍﻟﻨﻮﻋﻲ ﻭﺍﻟﻜﻤﻲ ﻟﻠﻜﺎﻓﻴﲔ .(RP-HPLC) ﺷﺎﻣﺒﻮﺑﺘﻮﻇﻴﻒ ﺗﻘﻨﻴﺔ “ﺛﻜﺮ ﻓﻠﺮ” ﻭ“ﺑﻨﺎﺩﻭﻝ ﺍﻛﺴﱰﺍ”ﰲ ﻣﺴﺘﺤﴬﻳﻦ ﺻﻴﺪﻻﻧﻴﲔ ﳘﺎ ﺑﺎﻻﺳﻢ؛ ﺍﻗﺮﺍﺹ . ﻭﻛﺎﻥ ﻣﻌﺪﻝ (40:60)ﻃﺮﻕ ﺍﻟﻌﻤﻞ: ﺗﻢ ﺍﺳﺘﺨﺪﺍﻡ ﻃﺮﻳﻘﺔ ﺍﻟﻄﻮﺭ ﺍﻟﺜﺎﺑﺖ ﻣﻊ ﻧﺴﺒﺔ ﺛﺎﺑﺘﺔ ﻟﻠﻨﺎﻗﻞ ﻛﲈ ﰲ ﺍﳌﻴﺜﺎﻧﻮﻝ: ﺍﳌﺎﺀ ﻧﺎﻧﻮﻣﱰ. ﻭﻗﺪ ﺭﺳﻢ ﻣﻨﺤﻨﻰ ﺍﳌﻌﺎﻳﺮﺓ ﻋﻦ ﻃﺮﻳﻖ ﺍﺳﺘﺨﺪﺍﻡ ﺗﺮﻛﻴﺰﺍﺕ ﺍﻟﺘﺎﻟﻴﺔ 273 ﻣﻞ /ﺩﻗﻴﻘﺔ ﺑﻴﻨﲈ ﻛﺎﻥ ﺍﻟﻄﻮﻝ ﺍﳌﻮﺟﻲ 0.8ﺗﺪﻓﻖ ﻣﻴﻜﺮﻭﻏﺮﺍﻡ /ﻣﻞ. ﺗﻢ ﻓﺤﺺ ﻋﻴﻨﺎﺕ ﺳﺘﺔ ﻭﺛﻼﺛﲔ ﺣﺒﺔ ﺩﻭﺍﺀ ﲢﺘﻮﻱ ﻋﲆ ﺍﻟﻜﺎﻓﻴﲔ ﻛﲈ ﺍﺫﻳﺒﺖ ﰲ (0.0, 20, 40, 60 ﻭ80) ﻣﻴﻜﺮﻭﻣﱰ ﻓﻠﱰ ﻏﺸﺎﺀ ﺍﻟﻨﺎﻳﻠﻮﻥ ﺫﻱ ﺍﳊﻘﻨﺔ. ﻣﻦ ﻧﺎﺣﻴﺔ ﺃﺧﺮ￯ ﺗﻢ ﻓﺤﺺ ﺛﻼﺙ 0.22ﺍﳌﻴﺜﺎﻧﻮﻝ ﻭﺗﻢ ﺗﺼﻔﻴﺘﻬﺎ ﺑﻮﺳﺎﻃﺔ 0.22ﻋﻴﻨﺎﺕ ﻣﺴﺘﻘﻠﺔ ﻟﻠﺸﺎﻣﺒﻮﻭﺍﻟﺘﻲ ﲢﺘﻮﻱ ﻋﲆ ﺍﻟﻜﺎﻓﻴﲔ. ﻭﺧﻔﻔﺖ ﺍﻟﻌﻴﻨﺎﺕ، ﺭﺟﺖ، ﻭﺗﻢ ﺗﺼﻔﻴﺘﻬﺎ ﻣﻊ ﻣﺮﺷﺤﺎﺕ ﺣﻘﻨﺔ ﻭﻗﺪ ﻟﻮﺣﻈﺖ ﺍﻟﺒﻴﺎﻧﺎﺕ ﻭﺗﻢ ﺗﺴﺠﻴﻠﻬﺎ.(HPLC)ﻣﻴﻜﺮﻭﻣﱰ ﺫﺍﺕ ﻏﺸﺎﺀ ﺍﻟﻨﺎﻳﻠﻮﻥ. ﻭﺃﺧﲑﺍ، ﺗﻢ ﺣﻘﻦ ﺍﳌﺤﺎﻟﻴﻞ ﺍﳌﺨﻔﻔﺔ ﺇﱃ ﻧﺘﺎﺋﺞ: ﻭﻗﺪ ﻟﻮﺣﻆ ﺍﳌﺠﺎﻻﺕ ﺍﳌﻄﻠﻮﺑﺔ ﻟﺮﺳﻢ ﻣﻨﺤﻨﻰ ﺍﳌﻌﺎﻳﺮﺓ. ﻭﻗﺪ ﺗﻢ ﲢﺪﻳﺪ ﺫﺭﻭﺓ ﺍﻟﻜﺎﻓﻴﲔ ﰲ ﻛﻞ ﻋﻴﻨﺔ ﺻﻴﺪﻻﻧﻴﺔ. ﻭﺑﻨﺎﺀ ﻋﲆ ﺫﻟﻚ ﺗﻢ ﲢﺪﻳﺪ ﻛﻤﻴﺔ ﺍﻟﻘﻤﻢ ﺍﻟﺘﻲ ﺗﻢ ﲢﺪﻳﺪﻫﺎ. ﺗﻢ ﺍﻟﻌﺜﻮﺭ ﻋﲆ ﳏﺘﻮ￯ ﺍﻟﻜﺎﻓﻴﲔ ﰲ ﺃﻗﺮﺍﺹ ﲢﺘﻮﻱ ﻋﲆ ﻣﺎﺩﺓ .0.0047٪ ﻣﻠﻎ. ﰲ ﺣﲔ ﻟﻮﺣﻆ ﺗﺮﻛﻴﺰ ﺍﻟﻜﺎﻓﻴﲔ ﰲ ﺍﻟﺸﺎﻣﺒﻮ64,96ﺍﻟﻜﺎﻓﻴﲔ ﻟﺘﻜﻮﻥ ﺍﻟﻄﻮﺭ ﺍﻟﻌﻜﻮﺱ ﻟﻠﻘﻴﺎﺱ ﺍﻟﻨﻮﻋﻲ ﻭﺍﻟﻜﻤﻲ HPLC ﺍﳌﻨﺎﻗﺸﺔ ﻭﺍﻻﺳﺘﻨﺘﺎﺝ: ﰲ ﻫﺬﺍ ﺍﻟﻌﻤﻞ ﺗﻢ ﺍﻻﺳﺘﻔﺎﺩﺓ ﻣﻦ ﺗﻘﻨﻴﺔ ﺍﻟـ Thicker Fuller ﻭﺷﺎﻣﺒﻮﺍﻟـ Panadol® Extraﳌﺎﺩﺓ ﺍﻟﻜﺎﻓﻴﲔ ﰲ ﻣﺴﺘﺤﴬﻳﻦ ﺻﻴﺪﻻﻧﻴﲔ ﳐﺘﻠﻔﲔ ﳘﺎ ﺑﺎﻻﺳﻢ؛ ﺍﻗﺮﺍﺹ ﺍﻟـ ﻟﺪﺭﺟﺔ ﺃﻥ ﺃﻱ ﺩﻭﺍﺀ ﻣﻮﺟﻮﺩ ﺗﻘﺮﻳﺒﺎ ﻳﻤﻜﻦ (HPLC). ﻗﺪ ﻧﻀﺠﺖ ﺗﻘﻨﻴﺔ ﺍﻟﻜﺮﻭﻣﺎﺗﻮﻏﺮﺍﻓﻴﺎ ﺍﻟﺴﺎﺋﻠﺔ ﻋﺎﻟﻴﺔ ﺍﻷﺩﺍﺀ Hair® ﲢﻠﻴﻠﻪ ﺑﺎﺳﺘﺨﺪﺍﻡ ﺍﻟﻄﺮﻕ ﺍﻟﻘﺎﺋﻤﺔ ﺍﻟﺘﻲ ﻳﻤﻜﻦ ﺍﻟﻌﺜﻮﺭ ﻋﻠﻴﻬﺎ ﰲ ﺍﻻﺩﺑﻴﺎﺕ ﺍﻟﺘﺤﻠﻴﻠﻴﺔ. ﻟﻠﻌﻤﻞ ﺍﳌﺴﺘﻘﺒﲇ ﻳﻤﻜﻦ ﻓﺤﺺ ﺃﺩﻭﻳﺔ ﺃﺧﺮ￯ ﺃﻭﻳﻤﻜﻦ ﻓﺤﺺ ﺣﺘﻰ ﺍﻟﱰﻛﻴﺒﺎﺕ ﺫﺍﺕ ﺍﻷﺩﻭﻳﺔ ﺍﳌﺘﻌﺪﺩﺓ ﻣﻊ ﻫﺬﻩ ﺍﻟﺘﻘﻨﻴﺔ ﺍﻟﺪﻗﻴﻘﺔ ﻭﺍﳌﻀﺒﻮﻃﺔ.

Keywords

Caffeine --- RP-HPLC --- Panadol Extra Tablets --- Thicker Fuller Caffeinated Shampoo. --- ﻛﺎﻓﻴﲔ، ﺍﻟﻜﺮﻭﻣﺎﺗﻮﻏﺮﺍﻓﻴﺎ ﺍﻟﺴﺎﺋﻠﺔ ﻋﺎﻟﻴﺔ ﺍﻻﺩﺍﺀ-ﺍﻟﻄﻮﺭ ﺍﻟﻌﻜﻮﺱ، ﺃﻗﺮﺍﺹ ﺑﺎﻧﺎﺩﻭﻝ ﺍﻛﺴﱰﺍ، ﺷﺎﻣﺒﻮﺛﻜﺮ ﻓﻠﺮ ﺍﳌﺤﺘﻮﻱ ﻋﲆ ﺍﻟﻜﺎﻓﻴﲔ.