Isolation and Molecular study of Human Adenovirus

Abstract

Human samples of adenovirus were isolated from the period of March, 1st, 2015 to 28th of May 2015 from eye secretions of patients were diagnosed through rapid device test and real time pcr. Positive cases of human adenovirus were sixty nine represent (83.13%). Isolation process of adenovirus was successfully performed on chicken embryo fibroblast cell culture for 4 th passage for suspected samples obtained from (eye swab) as well as isolate from chick embryo [specific pathogen free (SPF)],who were diagnosed . The study groups were divided into three groups with different age and gender. The group of 16-30 years old , the highest percent of infection (55.04%) in comparison with other aged group followed by the group of 31-45 years old (31.88%) ; then, aged 5- 15 years old (13.04%). The males groups represented the highest number of infection with adenovirus in comparison with females groups represented by 44 males (63.76%).

Keywords

adenovirus, eye swab