Plantlets Growth of Bintje and EigenheimerPotato under salt stressin vitro

Abstract

This study was conducted at the plant tissue culture lab. located at the Department of Horticulture and Landscape Design Gardening ∕ College of Agriculture ∕ university of Kufa, to studysome changes which happens in the parametersof plantlets growth for two verities of Bintje and Eigenheimer to salt stress. different concentrations of NaClwere used 0,50,100 and 150 mM in MS media supplied by 1 mg . L-1 BA, 1 mg . L-1thiamine, 0.5 mg . L-1 pyridoxine, 100 mg . L-1nicotinic acid, 100 mg . L-1Myo- inositol, 30 g . L-1 sucrose, and 7 g . L-1agarfor 4 weeks. Result showed a significant effects to NaCl concentration on the growth characteristics to the potatoes cultivars(Eigenheimer and Bintje) microshoots. Increasing salt concentration resulted reducing in shoots length, shoot dry and fresh weights , leaves number, roots number, and roots length. Also, results showed that there was no significant difference between the cultivars in the length of plantlet while Eigenheimer cultivar was differed significantly from Bintje cultivar in shoot dry and fresh weights , roots number, and roots length. According to the interaction results between the cultivars and NaCl concentrations, the interaction between Bintje cultivar and 0 mMNaCl showed the highest values of shoots length and leaves number, and the interaction between Eigenheimer and 50 mMNaCl was recorded the highest values of shoot dry and fresh weights, and roots number, while the interaction between Eigenheimer and 100 mMNaCl was showed a higher rate of roots length. It can be concluded that, addingNaCl to the media gave negative vegetative growth, which means that it is an indicator for the ability of potato verities to stand salt stress for the following studies. The Eigenheimerveritywith wastolerant to NaCl than the Bintje verity.