Isolation and Identification of Fungi Associated with Date Palm Leaflet Blight Disease and its Chemical and Biological Control

Abstract

This study was carried out at the laboratories of Department of Plant Protection, College of Agriculture, Basrah University. The study aimed to detect the leaflet blight disease for different datepalm cultivars such as Chipchap, Sayer, Zehdi and Khodrawi at three regoins, Medainah, Nashwa and Abu Al-khaseeb. The result showed that the infection percent was 86.51, 83.61 and 76.03% at Medainah, Abu Al-khaseeb and Nashwa respectively. The results also showed that the infection intensity was 25.96, 28.56 and 21.1 % for the three above regions respectively. The infection intensity also differs according to surfaces, it was 76.37 % on the lower surface , while it was 55.55 , 37.91 and 24.34% on the middle, foot and terminal of leaflet. while the other fungi caused yellowish and spotting symptoms sixteen species belonging to 13 genera were isolated from the leaflets , these species were as follows: Chaetomium atrobrunneum, C. globosum , Chalaropsis thielavioides, C.radicicola, Stemphylium.sp., Bipolaris australis, Cladosporium herbarum, Nigrospora sphaerica, Diplodia phoenicis, Phoma glomerata, Fusarium verticillioides, Alternaria alternate, A. longipes, Coniochaete sacchareo, Melanospora sp., and S.phoenicis. Most of them caused blight disease..Among of them D. phoenicis and S. phoenicis gave scorch symptoms pathogenicity testing. The field experiment revealed that pa fluorescence reduced infection intensity to 50% , while Dazim biological agent Toharziannm treatment were the lowest treatment in reducing the infection intensity which reduced to 4.1 and 6.3 respectively.