Effect of Inoculation with Pseudomonas fluorescens , Azospirillum brasilense and Trichoderma harzianum in Some Characteristics of Growth and phytohormones in Zea mays Plant

Abstract

This experiment was conducted in plastic pots at autman 2015-2016 in order to study the effect of inoculation by Trichoderma harzianum ,Azosperillum brasilense,and Pseudomonas fluorescens and the double and triple interactions of them on indicators of growth ,content of nuterial elements,and plant hormones (IAA,GA3) of Zea mays plant which cultivated in soil of Ankawa area in Erbil ,The three isolations was cultivated and confirmed by biochemical tests .then the three isolations examanted for their capility on produce IAA.The results showed that the isolation of T.h was excelled in ability of produce IAA ,the concentrations of IAA produced by Trichoderma harzianum was (0.028mg/ml.After passed 45 days on germination ,the samples of plant were taken , The results showed that the highest concentration of IAA and GA3 were recorded at triple inoculation treatment (plant inoculated with A.b+P.f+T.h ) which reached (48.16 ,582) µg/ml of IAA and GA3 respectively.while in conrol treatment (without inoculation) were (25.38,422)µg/ml,Also the results showed that the single,double,and triple inoculation treatments caused significant increase in concentration of chlorophyll which was reached (3.295mg/g f.w) in triple inoculation treatment..while at the control treatment was 1.486 mg/g f.w., The highest concentration of nitrogen were recorded at triple inoculation treatment A.b+P.f+T.h which given 3.916%, ,and caused significant increase compared with control treatment which was given the lowest nitrogen concentration reached 2.943%, Also ,the results confirmed that triple and double inoculation caused signficant increase in phosphor concentrations which were (31.06, 32.03,33.01,46.60)% for treatments A.b+P.f , A.b+T.h , P.f+T.h , A.b+P.f+T.h respectively.