Antifungal Activity of Silver Nanoparticles Synthesized by Ttichoderma harizanum

Abstract

In this study, Antifungal potential of silver nanoparticles synthesized fromby filtrate Ttichoderma harizanum on different pathogenic fungi was investigated. 10 mL of filtrate Ttichoderma harizanum on was mixed with 90 mL of (1 mM ) and (2 mM) aqueous AgNO3 and heated at 70 °C for 10 min. A change color solution to dark brown color was observed .Characterization using UV-VIS spectrophotometery. The UV-Vis spectral analysis showed silver surface plasmon resonance band at (299.5 – 435.5) nm in 2 mM aqueous AgNO3 . Antifungal activity against three fungi was tested using well Poisoned food method. The synthesized silver nanoparticles efficiently inhibited various pathogenic fungi in a dose dependentThe approach of dark brown synthesis seems to be cost effective, eco-friendly and easy alternative to conventional methods of silver nanoparticles synthesis. The powerful bioactivity demonstrated by the synthesized silver nanoparticles leads towards the clinical use as antifungal.