Abstract

The inhibitory efficiency of flavonoid extract of black mustard seeds (Brassica nigra) in growth of some bacteria and fungi isolated from local and Imported beef carcasses H. H. Hashem Coll.of Vet. Med./ Univ of AL-Qadisiya A. H. AHamadaniColl of Med./ Univ of AL-QadisiyaI. F. Al–JawharyColl of Med./ Univ of AL-Qadisiya AbstractThis study was conducted to detect the microbial numbers and species that associated the local and imported beef carcasses and the sanitary conditions for the slaughterhouses and the butcher’s shops in Al-Diwaniya city, and the possibility of illness germs existence that are connected with the food poisoning and gastroenteritis cases in human, like Staphylococcus aureus , Escherichia coli and other fungi like Penicillium notatum, Aspergillus spp. and others, in one hundred and sixty samples of beef carcasses (80 samples of local meat and 80 samples of imported meat) . The results showed that the total count of aerobic bacteria and Fungi for the beef carcasses when results statistically analysis under possibility level (P<0.05) show indicated of low significant differences between the local and imported meat , the local meat was more contamination than the imported meat , while the fungal contamination in imported meat was more than the local meat . Isolation and Identification of bacterial species from the local and imported meat and from the swabs which taken during this study , were Staphylococcus aureus (46.30 % in the local meat & 37.88 % in the imported meat ) , Escherichia coli (41.94 % in the local meat & 43.17 % in the Imported meat ) , Pseudomonas aeruginosa (9.73 % in the local meat & 12.77 % in the Imported meat ) and Proteus spp. (2.01% in the local meat & 3.96% in Imported meat) , while the most common fungi were Penicillium notatum (62.83% in the local meat & 73.77% in the Imported meat) , Aspergillus niger (24.77% in the local meat & 11.47% in the Imported meat ) , Alternaria alternata (7.07% in the local meat & 9.83% in the Imported meat) ,Aspergillus candidas (4.42% in the local meat ) , Aspergillus fumigatus (0.88% in the local meat & 3.27% in the Imported meat) and Aspergillus spp. (1.63% in the Imported meat ).The Flavonoid extract of Black Mustard seeds (Brassica nigra) show inhibition activity in growth of the following bacteria (Staphylococcus aureus , Pseudomonas aeruginosa) and fungi (Penicillum notatum , Aspergillus niger , Alternaria alternata ) and in all concentrations that uses (25,50,75,100) mg/ml , while Escherichia coli showed susceptibility in the concentration (75,100) mg/ ml and the results showed differential in the extract activity according to Microbial kinds that uses in this study that belong to species of bacteria and fungi and its structure. The Minimum Inhibition Concentration (MIC) of extract have been determined and in bacteria was (30) mg/ ml for each S. aureus and Ps. aeruginosa and (40) mg/ml for E. coli .and the Minimum bactericidal Concentration was (70)mg/ml for all bacteria which examined , while in fungi the minimum Inhibition concentration was (0.25) mg/ ml and the Minimum fungicidal Concentration was (5) mg/ml for all examined fungi

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