Distribution and localization of ground substance of carbohydrate group in an inflammatory and phenytion induced gingival enlargement using histochemical method

Abstract

ABSTRACT
Background: Gingival enlargement detected as a result of pathological changes or by induction of drugs such as
Phenytoin. Changes in distribution of macromolecules of glycogen, proteoglycan and glycoprotein in gingival
enlargement were observed by histochemical method. The aim of the study was to illustrate the localization and
distribution of ground substance in an inflammatory and Phenytoin induced gingival enlargement, using
histochemical methods.
Materials and Methods: Twenty two individuals, ten with inflammatory gingival enlargement, other ten with Phenytoin
induced gingival enlargement and two healthy person extraction of impacted 3rd molar as control .The specimens
were stained with periodic acid Schiff reagent (PAS).
Results: In the inflammatory gingival enlargement there is an increase in carbohydrate material production concern
to epithelial layer, basement membrane and underneath connective tissue showing reddish purple stain with PAS
reaction, while Phenytoin induced gingival enlargement showed increment in epithelial layer only.
Conclusion: Histochemical method by PAS stain used to show difference in distribution of carbohydrate group in
gingival specimens of inflammatory and Phenytoin induced gingival enlargement.
Key words: Gingival enlargement, Phenytoin, PAS stain. (J Bagh Coll Dentistry2009; 21(1): 49-52)