Study of extracted teichoich acid from Staphylococcus aureus isolated osteomylitis Patient

Abstract

This study deals with the isolation and identification of bacteria Staphylococcus aureus that cause osteomylitis ,and the extract teichoic acid from the wallof Staphylococcus aureus isolate and tried to identify the antibodies of teichoic acid in serum of patients complaining from osteomylitis.Thirty four samples of(pus and blood) were collected from hospitalized osteomylitis patient in BaqubaGeneral Hospital-Diyala province.The results of aerobic bacterial culture revealed 22 positive samples and 12 negative samples.The biochemical test revealed 9 isolates of Staphylococcus aureus .Extraction and partial purification of teichoic acid from the wall of Staphylococcus aureus was determind by using the mechanical disintegration procedure using speed shaking method to disintegrate the cell wall of Staphylococcus aureus by adding the glass beads and obtaining an appropriate amount from the disintegrate cells.Extaction and partial purifiacation of disintegrated wall depended on the digestion by proteolytic enzymes in addition to the use of sodium dodecyle sulphate (SDS)to removethe cytoplasmic membrane .The antibodies aginst cell wall of teichoic acid of Staphylococcus aureus were detected in the osteomylitis patient serum using immunodiffusion in gel test through the appearance of one precipitate band against teichoic acid extracted from the cell wall of Staphylococcus aureus