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Molecular detection of invA, ssaP in Salmonella typhimurium isolated from chicken in Al-Qadisiyah Province

Azhar Abdulsada Neama --- Jameela Radi Esmaeel --- Jinan Nadhim Sadeq

Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري
ISSN: 18185746 23134429 Year: 2017 Volume: 16 Issue: 2 Pages: 8-13
Publisher: Al-Qadisiyah University جامعة القادسية

Abstract

Salmonella is considered the most important cause of foodborne diseases. Identification of Salmonella typhimurium evaluated using bacteriological assay followed by PCR technique. In this study, 40 intestinal content specimens of poultry were collected randomly from different farms of Al-Qadisiyah province. Outs of 40 samples obtained, 14 isolates (35 %) were detected as Salmonella typhimurium according to conventional bacteriological characteristics, the Vitek 2 system for identification and molecular assays. Two sets of primers were designed for detecting invA and ssaP genes. These genes potent the virulence of Salmonella typhimurium. The primers were made in this study by using NCBIGenBank and design online. The primers were made by (Bioneer) company, Korea. Molecular assay of the isolates gives away specific PCR products of 677bp for the invA gene and 314bp for ssaP gene. The invA genes were amplified in 11 (78.5%) out of 14 isolates of Salmonella typhimurium, while ssaP genes were amplified in 10 (71.4%) out of 14 isolates of Salmonella typhimurium. The result of the study confirms the ability of these specific primers for detection of salmonella typhimurium in samples of chicken as well as the rapidly and sensitivity of the PCR method as a good tool for bacteriological identification.