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Detection and identification of the extended- spectrum β-lactamases (bla SHV and bla CTX –M) Klebsiella pneumonia by PCR technique

Zeena Fouad Saleh --- Moshtaq Talip Hussein --- Saba Falah Kleaf

Al-Qadisiyah Journal of Veterinary Medicine Sciences مجلة القادسية لعلوم الطب البيطري
ISSN: 18185746 23134429 Year: 2017 Volume: 16 Issue: 2 Pages: 22-28
Publisher: Al-Qadisiyah University جامعة القادسية

Abstract

Klebsiella pneumonia was the most important nosocomial infections pathogen. It was causing several morbidity and mortality in sick animals and human. Its identification and detection performed by usage of conventional cultural characters, biochemical tests and Polymerase chain reaction technique (PCR). One hundred clinical samples were divided into (50) samples of sheep suffering from pneumonia and (50) samples of a human with UTI, collected from different regions of Al-Diwaniyah city. Thirty-four (68%) sheep samples were positive for K. pneumonia identification, while 38 (76%) urine samples of a human with UTI cases gave K. pneumoniae isolates. The results show only (72) isolates were identified by PCR technique. Thirty isolates of human samples (78.9%) were positive for detection of bla CTX-M ESBLs, while its detection did not determine in sheep. Sixty-three isolates from total isolates were positive to detection of bla SHV, these positive isolates divided into 53 isolates for human and 28 sheep. Molecular characterization of ESBL provides information about the prevalence of ESBL producing K. pneumoniae in Al-Diwaniyah. The aim of this study was the determination of CTX-M and SHV genes presence in extended-spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae.