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Immunohistochemical Localization of Epstein Barr Virus- Latent Membrane Protein 1 in Breast Cancer Tissues

Saad Hasan Mohammed Ali *,Shakir H. Mohammed Al-Alwany**, Ghanim I.A. Al-Wadi *

Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية
ISSN: 16088360 Year: 2016 Volume: 15 Issue: 2 Pages: 234-240
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Abstract

ABSTRACT:BACK GROUND:Epstein- Barr virus (EBV) is well known to infect the vast majority of the world's adult population and has recently been reported in many literatures for its association with a broad spectrum of benign and malignant tumors, including breast cancer. OBJECTIVE: To analyze the relations and impact of Epstein Barr Virus- latent membrane protein 1(EBV-LMP 1) expression on a group of tissues with primary invasive breast cancers.PATIENTS AND METHODS: Forty- nine (49) formalin-fixed, paraffin- embedded breast tissues were obtained in this study; (34) biopsies from breast cancers (BC) and (15) from apparently normal breast autopsies control group. Detection of expression of Epstein Barr Virus- latent membrane protein 1(EBV-LMP 1) was done by HRP/DAB immunohistochemistry detection kit (an immunoenzymatic antigen detection system for immunohistochemistry techniques) using specific primary antibodies for EBV-LMP 1.RESULTS: Detection of EBV-LMP 1 - immunohistochemical (IHC) reactions in tissues with BC was observed in 11 out of 34 (32.4%), while in healthy breast tissues in the control group was detected in 13.3% (2 out of 15). The difference between the percentages of EBV-LMP 1 detection in BC tissues & control group was statistically not significant (P >0.05).While no significant differences were observed between EBV-LMP 1 detection in BC tissues & the age of patients as well as grade of invasive breast cancers (P value > 0.05).CONCLUSION: Our results indicate that EBV could have a role in the pathogenesis of subset of breast cancers and contribution in their carcinogenesis. .

Keywords

KEY WORD: breast cancer --- epstein barr virus --- ebv - latent membrane protein 1 --- immunohistochemical technique