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Immunohistochemical Evaluation of the Frequency of Human Papillomavirus in Cervical Lesions in a Sample from the North Iraqi Population

Ava Taher Ismael*, Rafal AbdulRazaq Al-Rawi*, Wahda M.T. AL-Nuaimy*

Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية
ISSN: 16088360 Year: 2016 Volume: 15 Issue: 2 Pages: 241-246
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Abstract

ABSTRACT:BACKGROUND: Human papilloma virus has been linked to many types of cervical lesions, ranging from the relatively innocuous lesions to fatal invasive squamous cell carcinoma. There is growing evidence of Human papillomavirus being a relevant factor in other anogenital cancers (anus, vulva, vagina and penis) as well as head and neck cancers.OBJECTIVE:To assess the feasibility of immunohistochemical staining paraffin sections for the presence of Human papilloma virus with monoclonal antibodies (clone K1H8, IgG) raised against the major coat fusion capsid proteins and to detect the frequency of human papilloma virus immunoexpression in benign, preneoplastic and neoplastic cervical lesions in patients living in Erbil city ( North of Iraq).MATERIALS AND METHODS: A total of 75 paraffin blocks samples of cervical tissue were retrieved retrospectively from the Pathology Department of Maternity Teaching Hospital and some private laboratories in Erbil city, during a period spanning from September 2013 to June 2014. They were categorized as: Benign cervicitis (10) samples, cervical intraepithelial neoplasia, CIN I (33) samples, CIN II (10) samples, CIN III (13) samples, cervical squamous cell carcinoma (6) samples and three samples with cervical adenocarcinoma Immunohistochemistry was performed on those samples using the avidin -biotin-peroxidase complex in which primarily monoclonal anti Human papilloma virus antibodies was used. RESULTS: None of the 10 samples of benign cervicitis were positive for Human papillomavirus protein while 21 out of 33 (63.6%) samples of CINI, 9 out of 10 (90%) samples of CIN II and 9 out of 13 (69.2%) samples of CIN III were positive for HPV. Also Human papillomavirus positivity observed in all six samples of squamous cell carcinoma (100%), mostly in sheets of less mature squamous cells and in 1 out of 3 (33.3%) samples of adenocarcinoma , mostly focal and in single cell.CONCLUSION: The immunohistochemical staining technique revealed a significant detection of HPV protein in cervical intraepithelial neoplasia and cervical carcinoma. .

Keywords

KEYWORDS: human papillomavirus --- immunohistochemistry --- cervical intraepithelial neoplasia --- cervical carcinoma