Molecular Detection the Agent that Causing Vaginitis in Vaginal Secretion from Women with Vaginitis and it Relation with Abortion

Abstract

Onehundred(100)swabswerecollectedfromwomenwithvaginitis,fromthehospitalof Babylon city and private clinics. All the samples were taken from married women from pregnancy. The age of patient (17– 40) years.The sample was collected by disposable swabs.Theseswabsstoredinfreezinguntilwereused,DNAwasextractedfromtheseswabs. The DNA genomic standard is prepared for the 16 S r RNA gene after the using of nanodropspectrophotometer(ng/ml)fordetectionagentcausingvaginitisbypolymerase chainreactiontechnique.TheresultappearedthatCandidaalbicanswasmorecommonin 79.17% while Trichomonas in 22.92%, other causes were bacterial vaginosis. Also studythe role of the infection agent with abortion,the result appeared that Megasphaera sp washigh risk with abortion.