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A study of p53 expression in transitional cell carcinoma of urinary bladder in Erbil governorate

دراسة تعبير p53 في سرطان الخلايا الانتقالية للمثانة في محافظة أربيل

Bakir Sadeq --- Salah A. Ali

Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية
ISSN: 19955588/19955596 Year: 2012 Volume: 16 Issue: 3 Pages: 248-255
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

Abstract

Background and objectives: This study aimed to evaluate p53 protein expression in both normal bladder epithelium and in cases of transitional cell carcinoma (TCC) of urinary blad-der by immunohistochemical study and to correlate p53 expression in urothelial cancer with other clinico-pathological parameters.Methods: This is a retrospective and prospective study for sample collection during the period from January 2006-May 2009. The samples studied included 105 formalin fixed, paraffin embedded urinary bladder tissue specimens; they consisted of the following diag-nostic categories: chronic non specific cystitis (n=5) and urothelial cancer (n=100). In this study the nuclear p53 protein expression was detected in tissue samples by Dako Cytoma-tion. LSAB + System-HRP staining protocol using monoclonal mouse anti human protein DO-7.Results: None of the chronic non specific cystitis cases showed p53 nuclear immunostain-ing, while 93% of urothelial cancer specimens examined showed immunopositivity for p53 protein. In this study, a statistically significant correlation was observed between p53 over-expression rate with the tumor grade (p= <0.001) and histological architecture (p= 0.023), but not with other clinico-pathological parameters like age and gender.Conclusion: Results of the present study showed the validity and simplicity of application of immunohistochemistry in determining the status of p53 protein expression. The results suggest that p53 overexpression is strongly associated with the aggressiveness of urothe-lial cancer.

Keywords

Urothelial carcinoma of urinary bladder --- P53