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Molecular Study of 16SrRNA Gene in Enterobacteriaceae isolated from Iraqi Patients

Ilham Abdul Hadi Khalaf --- Ahmed Mohamed Turkey --- Shahad Hisham Mahmood

Al-Nahrain Journal of Science مجلة النهرين للعلوم
ISSN: (print)26635453,(online)26635461 Year: 2018 Volume: 00 Issue: 1 Pages: 78-87
Publisher: Al-Nahrain University جامعة النهرين

Abstract

This study included the collection of 204 clinical and non-clinical samples from Ibn-Al-Baladi childbirth Hospital, Al-Yarmouk Teaching Hospital and Imam Ali Hospital in Baghdad, from both genders of different ages. The collected samples were distributed according to the collection source (urine, wounds, burns, feces, Tigris River water in Baghdad and soil samples). A total of 31 isolates of Escherichia coli (48.43 %), 15 isolates Klebsiella pneumoniae (23.43 %), 10 isolates of Enterobacter cloacae (15.62 %) and 8 isolates of proteus mirabilis (12.50 %) were isolated and identified based on microscopic culture, conventional methods, VITEK 2 and molecular identification of 16 SrRNA gene. Our investigations indicated that Escherichia coli and Klebsiella pneumoniae species represent the most frequent isolates, whereas both Enterobacter cloacae and Proteus mirabilis were the less frequent species. The results of the analysis of the sequencing of selected 21 isolates were deposited in the National Center for Biotechnology Information (NCBI) belong to the four species (From LC314477.1 to LC314497.1).

Keywords

Enterobacteriaceae --- 16S rRNA --- PCR --- Molecular Identification.